Expression of a chimaeric granule-bound starch synthase-GUS gene in transgenic potato plants View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-10

AUTHORS

Richard G. F. Visser, Arjan Stolte, Evert Jacobsen

ABSTRACT

Granule-bound starch synthase is the key enzyme in amylose synthesis. The regulation of this gene was investigated using a chimaeric gene consisting of a 0.8 kb 5' upstream sequence of the granule-bound starch synthase gene from potato and the beta-glucuronidase gene which was introduced into potato using an Agrobacterium tumefaciens binary vector system. The chimaeric gene was highly expressed in stolons and tubers, whereas the expression in leaves, stems or roots from greenhouse-grown plants was relatively low. However, leaves from in vitro grown plantlets exhibited an elevated GUS expression. The expression of the chimaeric gene was inducible in leaves by growth on relatively high concentrations of sucrose, fructose and glucose and was about 30- to 50-fold higher than in leaves from greenhouse-grown plants. The granule-bound starch synthase gene is expressed organ-specifically since stolons and tubers showed GUS activities 125- to 3350-fold higher than in leaves. The activities in these two organs are 3- to 25-fold higher than the expression of the CaMV-GUS gene. Histochemical analysis of different tissues showed that only certain regions of leaves and roots express high GUS activities. Stolons and tubers show high expression. More... »

PAGES

691-699

References to SciGraph publications

  • 1989-10. Efficient transformation of potato (Solanum tuberosum L.) using a binary vector in Agrobacterium rhizogenes in THEORETICAL AND APPLIED GENETICS
  • 1989-10. Sucrose-regulated expression of a chimeric potato tuber gene in leaves of transgenic tobacco plants in PLANT MOLECULAR BIOLOGY
  • 1990-04. Wound-inducible potato inhibitor II genes: enhancement of expression by sucrose in PLANT MOLECULAR BIOLOGY
  • 1989-01. Analysis of a chimeric class-I patatin-GUS gene in transgenic potato plants: High-level expression in tubers and sucrose-inducible expression in cultured leaf and stem explants in PLANT MOLECULAR BIOLOGY
  • 1990-06. Transcriptional regulation of a patatin-1 gene in potato in PLANT MOLECULAR BIOLOGY
  • 1987-12. Isolation of an amylose-free starch mutant of the potato (Solanum tuberosum L.) in THEORETICAL AND APPLIED GENETICS
  • 1989-03. Transformation of homozygous diploid potato with an Agrobacterium tumefaciens binary vector system by adventitious shoot regeneration on leaf and stem segments in PLANT MOLECULAR BIOLOGY
  • 1991-09. Complementation of the amylose-free starch mutant of potato (Solanum tuberosum.) by the gene encoding granule-bound starch synthase in THEORETICAL AND APPLIED GENETICS
  • 1990-09. Cis regulatory elements directing tuber-specific and sucrose-inducible expression of a chimeric class I patatin promoter/GUS-gene fusion in MOLECULAR GENETICS AND GENOMICS
  • 1990-10. One of two different ADP-glucose pyrophosphorylase genes from potato responds strongly to elevated levels of sucrose in MOLECULAR GENETICS AND GENOMICS
  • 1988-01. Introduction of foreign genes into potato cultivars Bintje and Désirée using an Agrobacterium tumefaciens binary vector in PLANT CELL REPORTS
  • Journal

    TITLE

    Plant Molecular Biology

    ISSUE

    4

    VOLUME

    17

    Author Affiliations

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00037054

    DOI

    http://dx.doi.org/10.1007/bf00037054

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1045905126

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/1912493


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