Synthesis and self-assembly of a functional monoclonal antibody in transgenic Nicotiana tabacum View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-08

AUTHORS

Klaus Düring, Sigrun Hippe, Fritz Kreuzaler, Jeff Schell

ABSTRACT

Immunoglobulin light and heavy chains are synthesized in mammalian cells as precursors containing a signal peptide. Processing and assembling result in formation of active antibodies. Chimeric genes have been made containing the coding sequence of the barley alpha-amylase signal peptide which has been fused to cDNAs coding for either the mature light or the mature heavy chain of a monoclonal antibody. A plasmid was constructed linking both chimeric genes under the control of plant active promoters in an expression cassette. This DNA fragment was stably integrated into the genome of Nicotiana tabacum by Agrobacterium tumefaciens mediated gene transfer. Synthesis of light and heavy chains and assembly to antibodies was detected in transgenic tobacco tissue using specific secondary antibodies. By electron microscopic immunogold labeling, the presence of assembled antibody could be detected within the endoplasmic reticulum. Affinity chromatography indicated biological activity of the assembled immunoglobulin produced in plant cells. Unexpectedly, a significant amount of assembled antibodies was found within chloroplasts. More... »

PAGES

281-293

Journal

TITLE

Plant Molecular Biology

ISSUE

2

VOLUME

15

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00036914

    DOI

    http://dx.doi.org/10.1007/bf00036914

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1014080521

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/2129424


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    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00036914'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00036914'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00036914'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00036914'


     

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    309 Max Planck Institute for Plant Breeding, D-5000, Köln, FRG
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    311 https://www.grid.ac/institutes/grid.4372.2 schema:alternateName Max Planck Society
    312 schema:name Max Planck Institute for Plant Breeding, D-5000, Köln, FRG
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