Synthesis and self-assembly of a functional monoclonal antibody in transgenic Nicotiana tabacum View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-08

AUTHORS

Klaus Düring, Sigrun Hippe, Fritz Kreuzaler, Jeff Schell

ABSTRACT

Immunoglobulin light and heavy chains are synthesized in mammalian cells as precursors containing a signal peptide. Processing and assembling result in formation of active antibodies. Chimeric genes have been made containing the coding sequence of the barley alpha-amylase signal peptide which has been fused to cDNAs coding for either the mature light or the mature heavy chain of a monoclonal antibody. A plasmid was constructed linking both chimeric genes under the control of plant active promoters in an expression cassette. This DNA fragment was stably integrated into the genome of Nicotiana tabacum by Agrobacterium tumefaciens mediated gene transfer. Synthesis of light and heavy chains and assembly to antibodies was detected in transgenic tobacco tissue using specific secondary antibodies. By electron microscopic immunogold labeling, the presence of assembled antibody could be detected within the endoplasmic reticulum. Affinity chromatography indicated biological activity of the assembled immunoglobulin produced in plant cells. Unexpectedly, a significant amount of assembled antibodies was found within chloroplasts. More... »

PAGES

281-293

Journal

TITLE

Plant Molecular Biology

ISSUE

2

VOLUME

15

Related Patents

  • Application Of Α-Amylase Gene Promoter And Signal Sequence In The Production Of Recombinant Proteins In Transgenic Plants And Transgenic Plant Seeds
  • Commercial Production Of Aprotinin In Plants
  • Commercial Production Of Avidin In Plants
  • Production Of Heterologous Proteins In Plants And Plant Cells
  • Zinc Finger Proteins For Dna Binding And Gene Regulation In Plants
  • Protein Production In Transgenic Alfalfa Plants
  • Immunoadhesin Comprising A Chimeric Icam-1 Molecule Produced In A Plant
  • Production Of Secreted Foreign Polypeptides In Plant Cell Culture
  • Method For Producing Immunoglobulins Containing Protection Proteins And Their Use
  • Immunoglobulin Binding Protein Arrays In Eukaryotic Cells
  • Trans-Activation Of Transcription From Viral Rna
  • Zinc Finger Proteins For Dna Binding And Gene Regulation In Plants
  • Method For Producing Immunoglobulins Containing Protection Proteins In Plants And Their Use
  • Aarobacterium-Mediated Method For Transforming Rice
  • Commercial Production Of Avidin In Plants
  • Immunoglobulins Containing Protection Proteins And Their Use
  • Plant Trichome Gland Specific Promoter Sequence
  • Transgenic Plants Expressing Assembled Secretory Antibodies
  • Commercial Production Of Avidin In Plants
  • Production Of Glycoproteins With Reduced O-Glycosylation
  • Zinc Finger Proteins For Dna Binding And Gene Regulation In Plants
  • Production Of Glycoproteins With Reduced O-Glycosylation
  • Expression Vectors For Concentrating A Recombinantly Produced Protein In Different Cell Compartments
  • Commercial Production Of Aprotinin In Plants
  • Process To Produce Engineered Antibodies In Plants, Engineered Antibodies And Use Thereof In Diagnostics And Therapeutics
  • Production Of Glycoproteins With Reduced O-Glycosylation
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00036914

    DOI

    http://dx.doi.org/10.1007/bf00036914

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1014080521

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/2129424


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Amino Acid Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Antibodies, Anti-Idiotypic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Antibodies, Monoclonal", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cloning, Molecular", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genes, Immunoglobulin", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genes, Plant", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genes, Synthetic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Vectors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Hordeum", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Immunoglobulin Heavy Chains", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Immunoglobulin Light Chains", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Immunohistochemistry", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Molecular Sequence Data", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plant Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plants, Toxic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein Sorting Signals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Recombinant Fusion Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Rhizobium", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Subcellular Fractions", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Tobacco", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "alpha-Amylases", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Max Planck Society", 
              "id": "https://www.grid.ac/institutes/grid.4372.2", 
              "name": [
                "Max Planck Institute for Plant Breeding, D-5000, K\u00f6ln, FRG"
              ], 
              "type": "Organization"
            }, 
            "familyName": "D\u00fcring", 
            "givenName": "Klaus", 
            "id": "sg:person.01021177634.45", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01021177634.45"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "RWTH Aachen University", 
              "id": "https://www.grid.ac/institutes/grid.1957.a", 
              "name": [
                "Institute for Biology III, RWTH Aachen, D-5100, Aachen, FRG"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hippe", 
            "givenName": "Sigrun", 
            "id": "sg:person.0764342061.09", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0764342061.09"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "RWTH Aachen University", 
              "id": "https://www.grid.ac/institutes/grid.1957.a", 
              "name": [
                "Max Planck Institute for Plant Breeding, D-5000, K\u00f6ln, FRG", 
                "Institute for Biology I, RWTH Aachen, D-5100, Aachen, FRG"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kreuzaler", 
            "givenName": "Fritz", 
            "id": "sg:person.01046656327.32", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01046656327.32"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Max Planck Society", 
              "id": "https://www.grid.ac/institutes/grid.4372.2", 
              "name": [
                "Max Planck Institute for Plant Breeding, D-5000, K\u00f6ln, FRG"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Schell", 
            "givenName": "Jeff", 
            "id": "sg:person.016705276677.96", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016705276677.96"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1016/0378-1119(84)90122-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000852526"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(84)90122-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000852526"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/314446a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003708008", 
              "https://doi.org/10.1038/314446a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.105.6.2665", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005743903"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/13.19.6981", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006386102"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/11.2.369", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007841328"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(81)90089-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009786716"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/227680a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010419937", 
              "https://doi.org/10.1038/227680a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(77)90074-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010881255"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(77)90074-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010881255"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.76.9.4350", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011704795"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/333330a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012301089", 
              "https://doi.org/10.1038/333330a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.85.7.2250", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015363449"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/333090a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016914459", 
              "https://doi.org/10.1038/333090a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(85)90120-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017120147"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(85)90120-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017120147"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0165-022x(84)90040-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017505209"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0165-022x(84)90040-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017505209"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.104.3.761", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017794114"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.105.6.2581", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018952991"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1186-991", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019022620", 
              "https://doi.org/10.1038/nbt1186-991"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.85.22.8678", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019050103"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.60.1.160", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023874808"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0022-2836(75)80083-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033296365"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.8.10.4250", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035975161"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.81.11.3273", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036454501"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/12.9.3791", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038281449"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/298380a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040142114", 
              "https://doi.org/10.1038/298380a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/342076a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040668704", 
              "https://doi.org/10.1038/342076a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(84)90076-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040910941"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(84)90076-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040910941"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/306387a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040976569", 
              "https://doi.org/10.1038/306387a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(77)90000-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044383550"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.102.5.1558", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047133765"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.106.4.1093", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050608153"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(82)90015-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050786151"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(82)90015-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050786151"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1105/tpc.1.11.1035", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051884737"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.3285470", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062604296"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.3285471", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062604297"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1983.tb01438.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1075014778"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1985.tb02312.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1077057833"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1983.tb01715.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1077183620"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1990.tb08085.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1078390056"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1081611031", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082180962", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1990-08", 
        "datePublishedReg": "1990-08-01", 
        "description": "Immunoglobulin light and heavy chains are synthesized in mammalian cells as precursors containing a signal peptide. Processing and assembling result in formation of active antibodies. Chimeric genes have been made containing the coding sequence of the barley alpha-amylase signal peptide which has been fused to cDNAs coding for either the mature light or the mature heavy chain of a monoclonal antibody. A plasmid was constructed linking both chimeric genes under the control of plant active promoters in an expression cassette. This DNA fragment was stably integrated into the genome of Nicotiana tabacum by Agrobacterium tumefaciens mediated gene transfer. Synthesis of light and heavy chains and assembly to antibodies was detected in transgenic tobacco tissue using specific secondary antibodies. By electron microscopic immunogold labeling, the presence of assembled antibody could be detected within the endoplasmic reticulum. Affinity chromatography indicated biological activity of the assembled immunoglobulin produced in plant cells. Unexpectedly, a significant amount of assembled antibodies was found within chloroplasts.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/bf00036914", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1101246", 
            "issn": [
              "0167-4412", 
              "1573-5028"
            ], 
            "name": "Plant Molecular Biology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "2", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "15"
          }
        ], 
        "name": "Synthesis and self-assembly of a functional monoclonal antibody in transgenic Nicotiana tabacum", 
        "pagination": "281-293", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "43140212af5b6454179f981e51fb282908f35106a2df0f292bdb087fcd11b59b"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "2129424"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9106343"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/bf00036914"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1014080521"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/bf00036914", 
          "https://app.dimensions.ai/details/publication/pub.1014080521"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T13:59", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130826_00000001.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007/BF00036914"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00036914'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00036914'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00036914'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00036914'


     

    This table displays all metadata directly associated to this object as RDF triples.

    313 TRIPLES      21 PREDICATES      92 URIs      44 LITERALS      32 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/bf00036914 schema:about N027c55ee565c4967a2cbf740f4b78f5d
    2 N09f715133c924071b9bcf48a50b73412
    3 N11ff06ddea0f46bea72aa7c145b9d46b
    4 N12aa5fe0ae4a40dbbac446d52ae24318
    5 N2776bfaf504a450aab7e5ea2c3b15970
    6 N2f52d66f5f904ae1a2cc0158f1933b55
    7 N2f8ab46ed55a45b5be258e10569b3f13
    8 N4c59a012176f40048b2913aec4871703
    9 N57c55f7205e64d10813bab18ebe95c8a
    10 N5b332d9ebb1641219266c2e10622b509
    11 N6abb323835164f4d8416f6d5b00cd288
    12 N6cb368552b904fbba405920b9cd8cfc5
    13 N970949085e2a4bb8939d44b7df6cf749
    14 N9dc31ea07e284883a78f4b52f5de189c
    15 Nb764eaa7da35417199dcdc23ac7c7c93
    16 Nb8b76e51b1e54300b7d5a28bb1f93724
    17 Nb902dbcb5cfc4f32bdb9b2dc20030b93
    18 Nb91f9613880c482298886689feaafc36
    19 Nc3c9bc428b864814ac4e768f3872324f
    20 Ncff88bb1b6c34574b24698ff7c22626b
    21 Nd203000462b44cb1bbd25081ea6abf8f
    22 Nd3434d941dff4a818b9cf803feb71730
    23 Nf97807a463ed4293aaa8287fc95618fb
    24 anzsrc-for:06
    25 anzsrc-for:0604
    26 schema:author N6c43d5b9107f424cb7c25c69b2544dd8
    27 schema:citation sg:pub.10.1038/227680a0
    28 sg:pub.10.1038/298380a0
    29 sg:pub.10.1038/306387a0
    30 sg:pub.10.1038/314446a0
    31 sg:pub.10.1038/333090a0
    32 sg:pub.10.1038/333330a0
    33 sg:pub.10.1038/342076a0
    34 sg:pub.10.1038/nbt1186-991
    35 https://app.dimensions.ai/details/publication/pub.1081611031
    36 https://app.dimensions.ai/details/publication/pub.1082180962
    37 https://doi.org/10.1002/j.1460-2075.1983.tb01438.x
    38 https://doi.org/10.1002/j.1460-2075.1983.tb01715.x
    39 https://doi.org/10.1002/j.1460-2075.1985.tb02312.x
    40 https://doi.org/10.1002/j.1460-2075.1990.tb08085.x
    41 https://doi.org/10.1016/0092-8674(81)90089-1
    42 https://doi.org/10.1016/0165-022x(84)90040-x
    43 https://doi.org/10.1016/0378-1119(77)90000-2
    44 https://doi.org/10.1016/0378-1119(77)90074-9
    45 https://doi.org/10.1016/0378-1119(82)90015-4
    46 https://doi.org/10.1016/0378-1119(84)90076-3
    47 https://doi.org/10.1016/0378-1119(84)90122-7
    48 https://doi.org/10.1016/0378-1119(85)90120-9
    49 https://doi.org/10.1016/s0022-2836(75)80083-0
    50 https://doi.org/10.1073/pnas.60.1.160
    51 https://doi.org/10.1073/pnas.76.9.4350
    52 https://doi.org/10.1073/pnas.81.11.3273
    53 https://doi.org/10.1073/pnas.85.22.8678
    54 https://doi.org/10.1073/pnas.85.7.2250
    55 https://doi.org/10.1083/jcb.102.5.1558
    56 https://doi.org/10.1083/jcb.104.3.761
    57 https://doi.org/10.1083/jcb.105.6.2581
    58 https://doi.org/10.1083/jcb.105.6.2665
    59 https://doi.org/10.1083/jcb.106.4.1093
    60 https://doi.org/10.1093/nar/11.2.369
    61 https://doi.org/10.1093/nar/12.9.3791
    62 https://doi.org/10.1093/nar/13.19.6981
    63 https://doi.org/10.1105/tpc.1.11.1035
    64 https://doi.org/10.1126/science.3285470
    65 https://doi.org/10.1126/science.3285471
    66 https://doi.org/10.1128/mcb.8.10.4250
    67 schema:datePublished 1990-08
    68 schema:datePublishedReg 1990-08-01
    69 schema:description Immunoglobulin light and heavy chains are synthesized in mammalian cells as precursors containing a signal peptide. Processing and assembling result in formation of active antibodies. Chimeric genes have been made containing the coding sequence of the barley alpha-amylase signal peptide which has been fused to cDNAs coding for either the mature light or the mature heavy chain of a monoclonal antibody. A plasmid was constructed linking both chimeric genes under the control of plant active promoters in an expression cassette. This DNA fragment was stably integrated into the genome of Nicotiana tabacum by Agrobacterium tumefaciens mediated gene transfer. Synthesis of light and heavy chains and assembly to antibodies was detected in transgenic tobacco tissue using specific secondary antibodies. By electron microscopic immunogold labeling, the presence of assembled antibody could be detected within the endoplasmic reticulum. Affinity chromatography indicated biological activity of the assembled immunoglobulin produced in plant cells. Unexpectedly, a significant amount of assembled antibodies was found within chloroplasts.
    70 schema:genre research_article
    71 schema:inLanguage en
    72 schema:isAccessibleForFree false
    73 schema:isPartOf N49053b2bc6a042e7ad79145667cefc1f
    74 Ndb8aab34f8a1459db8dc95221782d8ce
    75 sg:journal.1101246
    76 schema:name Synthesis and self-assembly of a functional monoclonal antibody in transgenic Nicotiana tabacum
    77 schema:pagination 281-293
    78 schema:productId N27fb88e151c148df8ddbe4be2e146499
    79 N7f51653b4c904e22b556f628f496dd91
    80 N9e05fe6465ec4c85b69f3d667b1f8b3d
    81 Ne6d1c221078d4d35b007824d5791c68c
    82 Ne9b3df576cb944e1bd4d7ff709ee1d7a
    83 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014080521
    84 https://doi.org/10.1007/bf00036914
    85 schema:sdDatePublished 2019-04-11T13:59
    86 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    87 schema:sdPublisher Nd225093ff8e44a07896c22cd3da45a01
    88 schema:url http://link.springer.com/10.1007/BF00036914
    89 sgo:license sg:explorer/license/
    90 sgo:sdDataset articles
    91 rdf:type schema:ScholarlyArticle
    92 N027c55ee565c4967a2cbf740f4b78f5d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    93 schema:name Antibodies, Anti-Idiotypic
    94 rdf:type schema:DefinedTerm
    95 N09f715133c924071b9bcf48a50b73412 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    96 schema:name Genes, Immunoglobulin
    97 rdf:type schema:DefinedTerm
    98 N11ff06ddea0f46bea72aa7c145b9d46b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    99 schema:name Recombinant Fusion Proteins
    100 rdf:type schema:DefinedTerm
    101 N12aa5fe0ae4a40dbbac446d52ae24318 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name Antibodies, Monoclonal
    103 rdf:type schema:DefinedTerm
    104 N2776bfaf504a450aab7e5ea2c3b15970 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    105 schema:name Genes, Synthetic
    106 rdf:type schema:DefinedTerm
    107 N27fb88e151c148df8ddbe4be2e146499 schema:name nlm_unique_id
    108 schema:value 9106343
    109 rdf:type schema:PropertyValue
    110 N2f52d66f5f904ae1a2cc0158f1933b55 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    111 schema:name Hordeum
    112 rdf:type schema:DefinedTerm
    113 N2f8ab46ed55a45b5be258e10569b3f13 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    114 schema:name Immunoglobulin Heavy Chains
    115 rdf:type schema:DefinedTerm
    116 N4744900beb574829a8d25f78cc55465b rdf:first sg:person.0764342061.09
    117 rdf:rest N4ca18be562644eeca8bd0ebfc291c989
    118 N49053b2bc6a042e7ad79145667cefc1f schema:issueNumber 2
    119 rdf:type schema:PublicationIssue
    120 N4c59a012176f40048b2913aec4871703 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    121 schema:name Rhizobium
    122 rdf:type schema:DefinedTerm
    123 N4ca18be562644eeca8bd0ebfc291c989 rdf:first sg:person.01046656327.32
    124 rdf:rest Necea6dc1654d4bf79176ead8e3ff8367
    125 N57c55f7205e64d10813bab18ebe95c8a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    126 schema:name Protein Sorting Signals
    127 rdf:type schema:DefinedTerm
    128 N5b332d9ebb1641219266c2e10622b509 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    129 schema:name Gene Expression
    130 rdf:type schema:DefinedTerm
    131 N6abb323835164f4d8416f6d5b00cd288 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    132 schema:name Amino Acid Sequence
    133 rdf:type schema:DefinedTerm
    134 N6c43d5b9107f424cb7c25c69b2544dd8 rdf:first sg:person.01021177634.45
    135 rdf:rest N4744900beb574829a8d25f78cc55465b
    136 N6cb368552b904fbba405920b9cd8cfc5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    137 schema:name Genetic Vectors
    138 rdf:type schema:DefinedTerm
    139 N7f51653b4c904e22b556f628f496dd91 schema:name doi
    140 schema:value 10.1007/bf00036914
    141 rdf:type schema:PropertyValue
    142 N970949085e2a4bb8939d44b7df6cf749 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    143 schema:name Tobacco
    144 rdf:type schema:DefinedTerm
    145 N9dc31ea07e284883a78f4b52f5de189c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    146 schema:name Subcellular Fractions
    147 rdf:type schema:DefinedTerm
    148 N9e05fe6465ec4c85b69f3d667b1f8b3d schema:name pubmed_id
    149 schema:value 2129424
    150 rdf:type schema:PropertyValue
    151 Nb764eaa7da35417199dcdc23ac7c7c93 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    152 schema:name Immunohistochemistry
    153 rdf:type schema:DefinedTerm
    154 Nb8b76e51b1e54300b7d5a28bb1f93724 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    155 schema:name Molecular Sequence Data
    156 rdf:type schema:DefinedTerm
    157 Nb902dbcb5cfc4f32bdb9b2dc20030b93 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    158 schema:name Genes, Plant
    159 rdf:type schema:DefinedTerm
    160 Nb91f9613880c482298886689feaafc36 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    161 schema:name Plants, Toxic
    162 rdf:type schema:DefinedTerm
    163 Nc3c9bc428b864814ac4e768f3872324f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    164 schema:name Plant Proteins
    165 rdf:type schema:DefinedTerm
    166 Ncff88bb1b6c34574b24698ff7c22626b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    167 schema:name alpha-Amylases
    168 rdf:type schema:DefinedTerm
    169 Nd203000462b44cb1bbd25081ea6abf8f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    170 schema:name Immunoglobulin Light Chains
    171 rdf:type schema:DefinedTerm
    172 Nd225093ff8e44a07896c22cd3da45a01 schema:name Springer Nature - SN SciGraph project
    173 rdf:type schema:Organization
    174 Nd3434d941dff4a818b9cf803feb71730 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    175 schema:name Cloning, Molecular
    176 rdf:type schema:DefinedTerm
    177 Ndb8aab34f8a1459db8dc95221782d8ce schema:volumeNumber 15
    178 rdf:type schema:PublicationVolume
    179 Ne6d1c221078d4d35b007824d5791c68c schema:name readcube_id
    180 schema:value 43140212af5b6454179f981e51fb282908f35106a2df0f292bdb087fcd11b59b
    181 rdf:type schema:PropertyValue
    182 Ne9b3df576cb944e1bd4d7ff709ee1d7a schema:name dimensions_id
    183 schema:value pub.1014080521
    184 rdf:type schema:PropertyValue
    185 Necea6dc1654d4bf79176ead8e3ff8367 rdf:first sg:person.016705276677.96
    186 rdf:rest rdf:nil
    187 Nf97807a463ed4293aaa8287fc95618fb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    188 schema:name Base Sequence
    189 rdf:type schema:DefinedTerm
    190 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    191 schema:name Biological Sciences
    192 rdf:type schema:DefinedTerm
    193 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    194 schema:name Genetics
    195 rdf:type schema:DefinedTerm
    196 sg:journal.1101246 schema:issn 0167-4412
    197 1573-5028
    198 schema:name Plant Molecular Biology
    199 rdf:type schema:Periodical
    200 sg:person.01021177634.45 schema:affiliation https://www.grid.ac/institutes/grid.4372.2
    201 schema:familyName Düring
    202 schema:givenName Klaus
    203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01021177634.45
    204 rdf:type schema:Person
    205 sg:person.01046656327.32 schema:affiliation https://www.grid.ac/institutes/grid.1957.a
    206 schema:familyName Kreuzaler
    207 schema:givenName Fritz
    208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01046656327.32
    209 rdf:type schema:Person
    210 sg:person.016705276677.96 schema:affiliation https://www.grid.ac/institutes/grid.4372.2
    211 schema:familyName Schell
    212 schema:givenName Jeff
    213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016705276677.96
    214 rdf:type schema:Person
    215 sg:person.0764342061.09 schema:affiliation https://www.grid.ac/institutes/grid.1957.a
    216 schema:familyName Hippe
    217 schema:givenName Sigrun
    218 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0764342061.09
    219 rdf:type schema:Person
    220 sg:pub.10.1038/227680a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010419937
    221 https://doi.org/10.1038/227680a0
    222 rdf:type schema:CreativeWork
    223 sg:pub.10.1038/298380a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040142114
    224 https://doi.org/10.1038/298380a0
    225 rdf:type schema:CreativeWork
    226 sg:pub.10.1038/306387a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040976569
    227 https://doi.org/10.1038/306387a0
    228 rdf:type schema:CreativeWork
    229 sg:pub.10.1038/314446a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003708008
    230 https://doi.org/10.1038/314446a0
    231 rdf:type schema:CreativeWork
    232 sg:pub.10.1038/333090a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016914459
    233 https://doi.org/10.1038/333090a0
    234 rdf:type schema:CreativeWork
    235 sg:pub.10.1038/333330a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012301089
    236 https://doi.org/10.1038/333330a0
    237 rdf:type schema:CreativeWork
    238 sg:pub.10.1038/342076a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040668704
    239 https://doi.org/10.1038/342076a0
    240 rdf:type schema:CreativeWork
    241 sg:pub.10.1038/nbt1186-991 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019022620
    242 https://doi.org/10.1038/nbt1186-991
    243 rdf:type schema:CreativeWork
    244 https://app.dimensions.ai/details/publication/pub.1081611031 schema:CreativeWork
    245 https://app.dimensions.ai/details/publication/pub.1082180962 schema:CreativeWork
    246 https://doi.org/10.1002/j.1460-2075.1983.tb01438.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1075014778
    247 rdf:type schema:CreativeWork
    248 https://doi.org/10.1002/j.1460-2075.1983.tb01715.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077183620
    249 rdf:type schema:CreativeWork
    250 https://doi.org/10.1002/j.1460-2075.1985.tb02312.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077057833
    251 rdf:type schema:CreativeWork
    252 https://doi.org/10.1002/j.1460-2075.1990.tb08085.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1078390056
    253 rdf:type schema:CreativeWork
    254 https://doi.org/10.1016/0092-8674(81)90089-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009786716
    255 rdf:type schema:CreativeWork
    256 https://doi.org/10.1016/0165-022x(84)90040-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1017505209
    257 rdf:type schema:CreativeWork
    258 https://doi.org/10.1016/0378-1119(77)90000-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044383550
    259 rdf:type schema:CreativeWork
    260 https://doi.org/10.1016/0378-1119(77)90074-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010881255
    261 rdf:type schema:CreativeWork
    262 https://doi.org/10.1016/0378-1119(82)90015-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050786151
    263 rdf:type schema:CreativeWork
    264 https://doi.org/10.1016/0378-1119(84)90076-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040910941
    265 rdf:type schema:CreativeWork
    266 https://doi.org/10.1016/0378-1119(84)90122-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000852526
    267 rdf:type schema:CreativeWork
    268 https://doi.org/10.1016/0378-1119(85)90120-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017120147
    269 rdf:type schema:CreativeWork
    270 https://doi.org/10.1016/s0022-2836(75)80083-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033296365
    271 rdf:type schema:CreativeWork
    272 https://doi.org/10.1073/pnas.60.1.160 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023874808
    273 rdf:type schema:CreativeWork
    274 https://doi.org/10.1073/pnas.76.9.4350 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011704795
    275 rdf:type schema:CreativeWork
    276 https://doi.org/10.1073/pnas.81.11.3273 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036454501
    277 rdf:type schema:CreativeWork
    278 https://doi.org/10.1073/pnas.85.22.8678 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019050103
    279 rdf:type schema:CreativeWork
    280 https://doi.org/10.1073/pnas.85.7.2250 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015363449
    281 rdf:type schema:CreativeWork
    282 https://doi.org/10.1083/jcb.102.5.1558 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047133765
    283 rdf:type schema:CreativeWork
    284 https://doi.org/10.1083/jcb.104.3.761 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017794114
    285 rdf:type schema:CreativeWork
    286 https://doi.org/10.1083/jcb.105.6.2581 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018952991
    287 rdf:type schema:CreativeWork
    288 https://doi.org/10.1083/jcb.105.6.2665 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005743903
    289 rdf:type schema:CreativeWork
    290 https://doi.org/10.1083/jcb.106.4.1093 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050608153
    291 rdf:type schema:CreativeWork
    292 https://doi.org/10.1093/nar/11.2.369 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007841328
    293 rdf:type schema:CreativeWork
    294 https://doi.org/10.1093/nar/12.9.3791 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038281449
    295 rdf:type schema:CreativeWork
    296 https://doi.org/10.1093/nar/13.19.6981 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006386102
    297 rdf:type schema:CreativeWork
    298 https://doi.org/10.1105/tpc.1.11.1035 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051884737
    299 rdf:type schema:CreativeWork
    300 https://doi.org/10.1126/science.3285470 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062604296
    301 rdf:type schema:CreativeWork
    302 https://doi.org/10.1126/science.3285471 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062604297
    303 rdf:type schema:CreativeWork
    304 https://doi.org/10.1128/mcb.8.10.4250 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035975161
    305 rdf:type schema:CreativeWork
    306 https://www.grid.ac/institutes/grid.1957.a schema:alternateName RWTH Aachen University
    307 schema:name Institute for Biology I, RWTH Aachen, D-5100, Aachen, FRG
    308 Institute for Biology III, RWTH Aachen, D-5100, Aachen, FRG
    309 Max Planck Institute for Plant Breeding, D-5000, Köln, FRG
    310 rdf:type schema:Organization
    311 https://www.grid.ac/institutes/grid.4372.2 schema:alternateName Max Planck Society
    312 schema:name Max Planck Institute for Plant Breeding, D-5000, Köln, FRG
    313 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...