Agrobacterium-mediated plant transformation by novel mini-T vectors in conjunction with a high-copy vir region helper plasmid View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-08

AUTHORS

Eva Zyprian, Clarence I. Kado

ABSTRACT

A new binary vector system for Agrobacterium-mediated plant transformation was developed. A set of four mini-T vectors comprised of T-DNA border sequences from nopaline-type Ti-plasmid pTiC58 flanking a chimaeric hygromycin-resistance gene for selection of transformants and up to eight unique restriction sites for cloning foreign DNA was constructed on a broad-host replicon containing the oriV of plasmid pSa. In two of the constructs these multiple cloning sites are flanked by a strong promoter to activate transcription of inserted DNA in planta. High-efficiency transformation was prompted by a high-copy, stable virulence helper plasmid pUCD2614, which contains a cloned virulence region of pTiC58 and tandem copies of the par locus of plasmid pTAR. Southern blot hybridization and genetic analyses of the progeny of transformed plants showed that the hygromycin resistance gene was stably inherited. More... »

PAGES

245-256

References to SciGraph publications

  • 1989-10. Efficient transformation of potato (Solanum tuberosum L.) using a binary vector in Agrobacterium rhizogenes in THEORETICAL AND APPLIED GENETICS
  • 1986-04. Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens in PLANT CELL REPORTS
  • 1985-03. Resistance to hygromycin B in PLANT MOLECULAR BIOLOGY
  • 1983-05-01. Mini–Ti: A New Vector Strategy for Plant Genetic Engineering in NATURE BIOTECHNOLOGY
  • 1988-12. High efficiency Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana leaf and cotyledon explants in PLANT CELL REPORTS
  • 1988-11. Genotype-independent leaf disc transformation of potato (Solanum tuberosum) using Agrobacterium tumefaciens in THEORETICAL AND APPLIED GENETICS
  • 1988. Regulation of Genes Involved in T-DNA Processing: An Initial Step in the Genetic Modification of Plant Cells in PLANT CELL BIOTECHNOLOGY
  • 1989-01. Specificity of Agrobacterium-mediated delivery of maize streak virus DNA to members of the Gramineae in PLANT MOLECULAR BIOLOGY
  • 1987-09. A simple and rapid method for minipreparation of DNA from tissue cultured plant cells in PLANT MOLECULAR BIOLOGY REPORTER
  • 1988. Design and Use of Agrobacterium Transformation Vectors in GENETIC ENGINEERING
  • 1985-09. A chimaeric hygromycin resistance gene as a selectable marker in plant cells in PLANT MOLECULAR BIOLOGY
  • 1983-05. A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid in NATURE
  • 1986-05. Bleomycin resistance: a new dominant selectable marker for plant cell transformation in PLANT MOLECULAR BIOLOGY
  • 1985-02. Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter in NATURE
  • 1987-12. Sequence context of the T-DNA border repeat element determines its relative activity during T-DNA transfer to plant cells in MOLECULAR GENETICS AND GENOMICS
  • 1987. Pathways to Plant Genetic Manipulation Employing Agrobacterium in PLANT DNA INFECTIOUS AGENTS
  • 1979-01. Isolation of a recombination deficient Agrobacterium tumefaciens mutant in MOLECULAR GENETICS AND GENOMICS
  • 1989-05. Virulence of Agrobacterium tumefaciens strains with Brassica napus and Brassica juncea in PLANT CELL REPORTS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00036911

    DOI

    http://dx.doi.org/10.1007/bf00036911

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1041352904

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/2103448


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