Transgene expression variability (position effect) of CAT and GUS reporter genes driven by linked divergent T-DNA promoters View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-07

AUTHORS

Cindy Peach, Jeff Velten

ABSTRACT

Forty-five individually transformed clonal tobacco callus lines were simultaneously assayed for both chloramphenicol acetyltransferase (CAT) and beta-glucuronidase (GUS) activity resulting from expression of introduced reporter genes driven by the adjacent and divergent mannopine (mas) promoters. Excluding lines in which one or both of the enzyme activities was essentially zero, the activities of the reporter genes varied by as much as a factor of 136 (CAT) and 175 (GUS) between individual transformants. Superimposed upon the high degree of inter-clonal expression variability was an intra-clonal variability of 3-4-fold. The observed degree of intra-clonal reporter gene activity may be more extreme because of the regulatory characteristics of the mannopine promoters, but must still be addressed when considering the limitations of reporter gene-based analysis of transgene function and structure. There was no consistent correlation between the expression levels of the introduced CAT and GUS genes since the ratio of GUS to CAT activities (nmol min-1 mg-1) within individual lines varied from 0.05 to 49. Even divergent transcription from two directly adjacent promoter regions (both contained within a 479 bp TR-DNA fragment) is insufficient to guarantee concurrent expression of two linked transgenes. Our quantitative data were compared to published data of transgene expression variability to examine the overall distribution of expression levels in individual transformants. The resulting frequency distribution indicates that most transformants express introduced transgenes at relatively low levels, suggesting that a potentially large number of Agrobacterium-mediated transformation events may result in silent transgenes. More... »

PAGES

49-60

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00036805

DOI

http://dx.doi.org/10.1007/bf00036805

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1000421689

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/1907871


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chloramphenicol O-Acetyltransferase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Clone Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cloning, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Bacterial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glucuronidase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mannitol", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plants, Toxic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Promoter Regions, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rhizobium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tobacco", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transformation, Genetic", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "New Mexico State University", 
          "id": "https://www.grid.ac/institutes/grid.24805.3b", 
          "name": [
            "Graduate Program in Molecular Biology, New Mexico State University, 88003-0001, Las Cruces, NM, USA", 
            "Chemistry Department, New Mexico State University, 88003-0001, Las Cruces, NM, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Peach", 
        "givenName": "Cindy", 
        "id": "sg:person.01111265466.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01111265466.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "New Mexico State University", 
          "id": "https://www.grid.ac/institutes/grid.24805.3b", 
          "name": [
            "Graduate Program in Molecular Biology, New Mexico State University, 88003-0001, Las Cruces, NM, USA", 
            "Plant Genetic Engineering Laboratory, New Mexico State University, 88003-0001, Las Cruces, NM, USA", 
            "Chemistry Department, New Mexico State University, 88003-0001, Las Cruces, NM, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Velten", 
        "givenName": "Jeff", 
        "id": "sg:person.01126535503.66", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01126535503.66"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1104/pp.81.1.86", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000555249"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-94-009-0951-9_1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003188633", 
          "https://doi.org/10.1007/978-94-009-0951-9_1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-94-009-0951-9_1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003188633", 
          "https://doi.org/10.1007/978-94-009-0951-9_1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00330568", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004762169", 
          "https://doi.org/10.1007/bf00330568"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00425708", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006156766", 
          "https://doi.org/10.1007/bf00425708"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/13.19.6981", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006386102"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00330420", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006658195", 
          "https://doi.org/10.1007/bf00330420"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.1.1.141", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009036462"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02428025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009806071", 
          "https://doi.org/10.1007/bf02428025"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02428025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009806071", 
          "https://doi.org/10.1007/bf02428025"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.86.9.3219", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009821741"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.2.4.279", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014921424"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.2.4.291", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017136927"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02667740", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017993505", 
          "https://doi.org/10.1007/bf02667740"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02667740", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017993505", 
          "https://doi.org/10.1007/bf02667740"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/15.24.10159", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018766354"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/16.15.7601", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019876717"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.83.6.1767", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022091054"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00331583", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022101620", 
          "https://doi.org/10.1007/bf00331583"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00025327", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022354452", 
          "https://doi.org/10.1007/bf00025327"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00025327", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022354452", 
          "https://doi.org/10.1007/bf00025327"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/newbio244029a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022855491", 
          "https://doi.org/10.1038/newbio244029a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/newbio244029a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022855491", 
          "https://doi.org/10.1038/newbio244029a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/newbio244029a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022855491", 
          "https://doi.org/10.1038/newbio244029a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4684-5377-5_3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022932440", 
          "https://doi.org/10.1007/978-1-4684-5377-5_3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00028779", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023339364", 
          "https://doi.org/10.1007/bf00028779"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00028779", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023339364", 
          "https://doi.org/10.1007/bf00028779"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1399-3054.1962.tb08052.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023703429"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/328203a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023828213", 
          "https://doi.org/10.1038/328203a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/292860a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024461091", 
          "https://doi.org/10.1038/292860a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0044-328x(76)80093-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024944233"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(76)90527-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025529346"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00752901", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025672137", 
          "https://doi.org/10.1007/bf00752901"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00752901", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025672137", 
          "https://doi.org/10.1007/bf00752901"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00033379", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028552579", 
          "https://doi.org/10.1007/bf00033379"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00033379", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028552579", 
          "https://doi.org/10.1007/bf00033379"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00338085", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032502861", 
          "https://doi.org/10.1007/bf00338085"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/277129a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032852748", 
          "https://doi.org/10.1038/277129a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-2836(75)80083-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033296365"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-94-009-2103-0_19", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034033269", 
          "https://doi.org/10.1007/978-94-009-2103-0_19"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-94-009-2103-0_19", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034033269", 
          "https://doi.org/10.1007/978-94-009-2103-0_19"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00027503", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035398678", 
          "https://doi.org/10.1007/bf00027503"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00027503", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035398678", 
          "https://doi.org/10.1007/bf00027503"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0065-2660(08)60040-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036596913"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00329940", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037063138", 
          "https://doi.org/10.1007/bf00329940"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(86)90762-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038072915"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/348749a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038802363", 
          "https://doi.org/10.1038/348749a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.82.19.6384", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040101303"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/12.5.2391", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040662176"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00331618", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041238099", 
          "https://doi.org/10.1007/bf00331618"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(87)90584-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041471577"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0168-9452(89)90121-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046326870"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.83.22.8447", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047987843"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/328248a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052533155", 
          "https://doi.org/10.1038/328248a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/328251a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053741745", 
          "https://doi.org/10.1038/328251a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/dna.1986.5.101", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059250789"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/16.19.9267", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059931705"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.232.4754.1106", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062532481"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2135/cropsci1978.0011183x001800060011x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069018052"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2307/3869070", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070467152"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2307/3869076", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070467156"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2307/3869077", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070467157"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1985.tb03949.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077060020"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077114457", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1983.tb01715.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077183620"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1984.tb02202.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077183717"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1985.tb03626.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077183746"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1985.tb04046.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077183792"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1987.tb02348.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077183914"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1989.tb03421.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077184027"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078652042", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1989.tb03466.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1079008175"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1989.tb03383.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1079178806"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079420313", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1987.tb04753.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1079517126"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081614666", 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-94-017-5294-7_3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1089711437", 
          "https://doi.org/10.1007/978-94-017-5294-7_3"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1991-07", 
    "datePublishedReg": "1991-07-01", 
    "description": "Forty-five individually transformed clonal tobacco callus lines were simultaneously assayed for both chloramphenicol acetyltransferase (CAT) and beta-glucuronidase (GUS) activity resulting from expression of introduced reporter genes driven by the adjacent and divergent mannopine (mas) promoters. Excluding lines in which one or both of the enzyme activities was essentially zero, the activities of the reporter genes varied by as much as a factor of 136 (CAT) and 175 (GUS) between individual transformants. Superimposed upon the high degree of inter-clonal expression variability was an intra-clonal variability of 3-4-fold. The observed degree of intra-clonal reporter gene activity may be more extreme because of the regulatory characteristics of the mannopine promoters, but must still be addressed when considering the limitations of reporter gene-based analysis of transgene function and structure. There was no consistent correlation between the expression levels of the introduced CAT and GUS genes since the ratio of GUS to CAT activities (nmol min-1 mg-1) within individual lines varied from 0.05 to 49. Even divergent transcription from two directly adjacent promoter regions (both contained within a 479 bp TR-DNA fragment) is insufficient to guarantee concurrent expression of two linked transgenes. Our quantitative data were compared to published data of transgene expression variability to examine the overall distribution of expression levels in individual transformants. The resulting frequency distribution indicates that most transformants express introduced transgenes at relatively low levels, suggesting that a potentially large number of Agrobacterium-mediated transformation events may result in silent transgenes.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf00036805", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2670449", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1101246", 
        "issn": [
          "0167-4412", 
          "1573-5028"
        ], 
        "name": "Plant Molecular Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "17"
      }
    ], 
    "name": "Transgene expression variability (position effect) of CAT and GUS reporter genes driven by linked divergent T-DNA promoters", 
    "pagination": "49-60", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "02019f1fbd2cd2a7cbfcf90ec8b9914bf3bb457db041682e81818ce9c385398f"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "1907871"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9106343"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00036805"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1000421689"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00036805", 
      "https://app.dimensions.ai/details/publication/pub.1000421689"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:54", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130811_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF00036805"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00036805'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00036805'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00036805'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00036805'


 

This table displays all metadata directly associated to this object as RDF triples.

347 TRIPLES      21 PREDICATES      107 URIs      33 LITERALS      21 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00036805 schema:about N01bd050d658a44bcb581bca26810fc23
2 N16dec95d2c084becbecdb9ca9050b04f
3 N1b10c081998740f8a6ec28dc55b55441
4 N1faaa50800ff4d96a3b03fde79c9044b
5 N55babc1db6c048ce8fac1c8740b8aad5
6 N6cd0d66680034f86a2a844cba1db3551
7 N6cfb6a8a7f384aa48cdf348c65e38b03
8 Nb1768c8fc06b425d88568e5dc9ecf289
9 Nb1d74cb57699441099f52a9f2c5ad098
10 Nc57be4fe48884d0fa3320130b7f9d921
11 Ndb52f9e87d40476592f1a2d80e468df7
12 Nf9a8fab14edd4b5ab0b5e3ea1baee4a3
13 anzsrc-for:06
14 anzsrc-for:0604
15 schema:author N84e71505e63d4d2faee32ab02f0870b7
16 schema:citation sg:pub.10.1007/978-1-4684-5377-5_3
17 sg:pub.10.1007/978-94-009-0951-9_1
18 sg:pub.10.1007/978-94-009-2103-0_19
19 sg:pub.10.1007/978-94-017-5294-7_3
20 sg:pub.10.1007/bf00025327
21 sg:pub.10.1007/bf00027503
22 sg:pub.10.1007/bf00028779
23 sg:pub.10.1007/bf00033379
24 sg:pub.10.1007/bf00329940
25 sg:pub.10.1007/bf00330420
26 sg:pub.10.1007/bf00330568
27 sg:pub.10.1007/bf00331583
28 sg:pub.10.1007/bf00331618
29 sg:pub.10.1007/bf00338085
30 sg:pub.10.1007/bf00425708
31 sg:pub.10.1007/bf00752901
32 sg:pub.10.1007/bf02428025
33 sg:pub.10.1007/bf02667740
34 sg:pub.10.1038/277129a0
35 sg:pub.10.1038/292860a0
36 sg:pub.10.1038/328203a0
37 sg:pub.10.1038/328248a0
38 sg:pub.10.1038/328251a0
39 sg:pub.10.1038/348749a0
40 sg:pub.10.1038/newbio244029a0
41 https://app.dimensions.ai/details/publication/pub.1077114457
42 https://app.dimensions.ai/details/publication/pub.1078652042
43 https://app.dimensions.ai/details/publication/pub.1079420313
44 https://app.dimensions.ai/details/publication/pub.1081614666
45 https://doi.org/10.1002/j.1460-2075.1983.tb01715.x
46 https://doi.org/10.1002/j.1460-2075.1984.tb02202.x
47 https://doi.org/10.1002/j.1460-2075.1985.tb03626.x
48 https://doi.org/10.1002/j.1460-2075.1985.tb03949.x
49 https://doi.org/10.1002/j.1460-2075.1985.tb04046.x
50 https://doi.org/10.1002/j.1460-2075.1987.tb02348.x
51 https://doi.org/10.1002/j.1460-2075.1987.tb04753.x
52 https://doi.org/10.1002/j.1460-2075.1989.tb03383.x
53 https://doi.org/10.1002/j.1460-2075.1989.tb03421.x
54 https://doi.org/10.1002/j.1460-2075.1989.tb03466.x
55 https://doi.org/10.1016/0003-2697(76)90527-3
56 https://doi.org/10.1016/0092-8674(86)90762-2
57 https://doi.org/10.1016/0092-8674(87)90584-8
58 https://doi.org/10.1016/0168-9452(89)90121-0
59 https://doi.org/10.1016/s0022-2836(75)80083-0
60 https://doi.org/10.1016/s0044-328x(76)80093-1
61 https://doi.org/10.1016/s0065-2660(08)60040-1
62 https://doi.org/10.1073/pnas.82.19.6384
63 https://doi.org/10.1073/pnas.83.22.8447
64 https://doi.org/10.1073/pnas.83.6.1767
65 https://doi.org/10.1073/pnas.86.9.3219
66 https://doi.org/10.1089/dna.1986.5.101
67 https://doi.org/10.1093/nar/12.5.2391
68 https://doi.org/10.1093/nar/13.19.6981
69 https://doi.org/10.1093/nar/15.24.10159
70 https://doi.org/10.1093/nar/16.15.7601
71 https://doi.org/10.1093/nar/16.19.9267
72 https://doi.org/10.1104/pp.81.1.86
73 https://doi.org/10.1105/tpc.1.1.141
74 https://doi.org/10.1105/tpc.2.4.279
75 https://doi.org/10.1105/tpc.2.4.291
76 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
77 https://doi.org/10.1126/science.232.4754.1106
78 https://doi.org/10.2135/cropsci1978.0011183x001800060011x
79 https://doi.org/10.2307/3869070
80 https://doi.org/10.2307/3869076
81 https://doi.org/10.2307/3869077
82 schema:datePublished 1991-07
83 schema:datePublishedReg 1991-07-01
84 schema:description Forty-five individually transformed clonal tobacco callus lines were simultaneously assayed for both chloramphenicol acetyltransferase (CAT) and beta-glucuronidase (GUS) activity resulting from expression of introduced reporter genes driven by the adjacent and divergent mannopine (mas) promoters. Excluding lines in which one or both of the enzyme activities was essentially zero, the activities of the reporter genes varied by as much as a factor of 136 (CAT) and 175 (GUS) between individual transformants. Superimposed upon the high degree of inter-clonal expression variability was an intra-clonal variability of 3-4-fold. The observed degree of intra-clonal reporter gene activity may be more extreme because of the regulatory characteristics of the mannopine promoters, but must still be addressed when considering the limitations of reporter gene-based analysis of transgene function and structure. There was no consistent correlation between the expression levels of the introduced CAT and GUS genes since the ratio of GUS to CAT activities (nmol min-1 mg-1) within individual lines varied from 0.05 to 49. Even divergent transcription from two directly adjacent promoter regions (both contained within a 479 bp TR-DNA fragment) is insufficient to guarantee concurrent expression of two linked transgenes. Our quantitative data were compared to published data of transgene expression variability to examine the overall distribution of expression levels in individual transformants. The resulting frequency distribution indicates that most transformants express introduced transgenes at relatively low levels, suggesting that a potentially large number of Agrobacterium-mediated transformation events may result in silent transgenes.
85 schema:genre research_article
86 schema:inLanguage en
87 schema:isAccessibleForFree false
88 schema:isPartOf N49edd8decf3f457cb98a5b6842cf655d
89 N7c1d7eb053b84e0ab974daf4ab107682
90 sg:journal.1101246
91 schema:name Transgene expression variability (position effect) of CAT and GUS reporter genes driven by linked divergent T-DNA promoters
92 schema:pagination 49-60
93 schema:productId N1bf85bc640ce4410a2e3f2ac6441b29c
94 N2d1b11f1df9744d1af757c0ed03779b8
95 N7a6f1b20dd77483f975427eeef02c99b
96 N9643d7abc4124852b2b78c0cde5c0551
97 Nf2e58342b6fa437396fd402ddbe06069
98 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000421689
99 https://doi.org/10.1007/bf00036805
100 schema:sdDatePublished 2019-04-11T13:54
101 schema:sdLicense https://scigraph.springernature.com/explorer/license/
102 schema:sdPublisher Naf649fa5f88e4515860772a0e997173b
103 schema:url http://link.springer.com/10.1007/BF00036805
104 sgo:license sg:explorer/license/
105 sgo:sdDataset articles
106 rdf:type schema:ScholarlyArticle
107 N01bd050d658a44bcb581bca26810fc23 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Tobacco
109 rdf:type schema:DefinedTerm
110 N16dec95d2c084becbecdb9ca9050b04f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Rhizobium
112 rdf:type schema:DefinedTerm
113 N1b10c081998740f8a6ec28dc55b55441 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Mannitol
115 rdf:type schema:DefinedTerm
116 N1bf85bc640ce4410a2e3f2ac6441b29c schema:name pubmed_id
117 schema:value 1907871
118 rdf:type schema:PropertyValue
119 N1faaa50800ff4d96a3b03fde79c9044b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Gene Expression Regulation
121 rdf:type schema:DefinedTerm
122 N2d1b11f1df9744d1af757c0ed03779b8 schema:name nlm_unique_id
123 schema:value 9106343
124 rdf:type schema:PropertyValue
125 N49edd8decf3f457cb98a5b6842cf655d schema:volumeNumber 17
126 rdf:type schema:PublicationVolume
127 N55babc1db6c048ce8fac1c8740b8aad5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Promoter Regions, Genetic
129 rdf:type schema:DefinedTerm
130 N6cd0d66680034f86a2a844cba1db3551 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Cloning, Molecular
132 rdf:type schema:DefinedTerm
133 N6cfb6a8a7f384aa48cdf348c65e38b03 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Glucuronidase
135 rdf:type schema:DefinedTerm
136 N7a6f1b20dd77483f975427eeef02c99b schema:name dimensions_id
137 schema:value pub.1000421689
138 rdf:type schema:PropertyValue
139 N7c1d7eb053b84e0ab974daf4ab107682 schema:issueNumber 1
140 rdf:type schema:PublicationIssue
141 N84e71505e63d4d2faee32ab02f0870b7 rdf:first sg:person.01111265466.05
142 rdf:rest Nb8a43fa60def428bb2eb18f245c15af5
143 N9643d7abc4124852b2b78c0cde5c0551 schema:name doi
144 schema:value 10.1007/bf00036805
145 rdf:type schema:PropertyValue
146 Naf649fa5f88e4515860772a0e997173b schema:name Springer Nature - SN SciGraph project
147 rdf:type schema:Organization
148 Nb1768c8fc06b425d88568e5dc9ecf289 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Transformation, Genetic
150 rdf:type schema:DefinedTerm
151 Nb1d74cb57699441099f52a9f2c5ad098 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Clone Cells
153 rdf:type schema:DefinedTerm
154 Nb8a43fa60def428bb2eb18f245c15af5 rdf:first sg:person.01126535503.66
155 rdf:rest rdf:nil
156 Nc57be4fe48884d0fa3320130b7f9d921 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Chloramphenicol O-Acetyltransferase
158 rdf:type schema:DefinedTerm
159 Ndb52f9e87d40476592f1a2d80e468df7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Plants, Toxic
161 rdf:type schema:DefinedTerm
162 Nf2e58342b6fa437396fd402ddbe06069 schema:name readcube_id
163 schema:value 02019f1fbd2cd2a7cbfcf90ec8b9914bf3bb457db041682e81818ce9c385398f
164 rdf:type schema:PropertyValue
165 Nf9a8fab14edd4b5ab0b5e3ea1baee4a3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
166 schema:name DNA, Bacterial
167 rdf:type schema:DefinedTerm
168 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
169 schema:name Biological Sciences
170 rdf:type schema:DefinedTerm
171 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
172 schema:name Genetics
173 rdf:type schema:DefinedTerm
174 sg:grant.2670449 http://pending.schema.org/fundedItem sg:pub.10.1007/bf00036805
175 rdf:type schema:MonetaryGrant
176 sg:journal.1101246 schema:issn 0167-4412
177 1573-5028
178 schema:name Plant Molecular Biology
179 rdf:type schema:Periodical
180 sg:person.01111265466.05 schema:affiliation https://www.grid.ac/institutes/grid.24805.3b
181 schema:familyName Peach
182 schema:givenName Cindy
183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01111265466.05
184 rdf:type schema:Person
185 sg:person.01126535503.66 schema:affiliation https://www.grid.ac/institutes/grid.24805.3b
186 schema:familyName Velten
187 schema:givenName Jeff
188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01126535503.66
189 rdf:type schema:Person
190 sg:pub.10.1007/978-1-4684-5377-5_3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022932440
191 https://doi.org/10.1007/978-1-4684-5377-5_3
192 rdf:type schema:CreativeWork
193 sg:pub.10.1007/978-94-009-0951-9_1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003188633
194 https://doi.org/10.1007/978-94-009-0951-9_1
195 rdf:type schema:CreativeWork
196 sg:pub.10.1007/978-94-009-2103-0_19 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034033269
197 https://doi.org/10.1007/978-94-009-2103-0_19
198 rdf:type schema:CreativeWork
199 sg:pub.10.1007/978-94-017-5294-7_3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1089711437
200 https://doi.org/10.1007/978-94-017-5294-7_3
201 rdf:type schema:CreativeWork
202 sg:pub.10.1007/bf00025327 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022354452
203 https://doi.org/10.1007/bf00025327
204 rdf:type schema:CreativeWork
205 sg:pub.10.1007/bf00027503 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035398678
206 https://doi.org/10.1007/bf00027503
207 rdf:type schema:CreativeWork
208 sg:pub.10.1007/bf00028779 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023339364
209 https://doi.org/10.1007/bf00028779
210 rdf:type schema:CreativeWork
211 sg:pub.10.1007/bf00033379 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028552579
212 https://doi.org/10.1007/bf00033379
213 rdf:type schema:CreativeWork
214 sg:pub.10.1007/bf00329940 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037063138
215 https://doi.org/10.1007/bf00329940
216 rdf:type schema:CreativeWork
217 sg:pub.10.1007/bf00330420 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006658195
218 https://doi.org/10.1007/bf00330420
219 rdf:type schema:CreativeWork
220 sg:pub.10.1007/bf00330568 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004762169
221 https://doi.org/10.1007/bf00330568
222 rdf:type schema:CreativeWork
223 sg:pub.10.1007/bf00331583 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022101620
224 https://doi.org/10.1007/bf00331583
225 rdf:type schema:CreativeWork
226 sg:pub.10.1007/bf00331618 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041238099
227 https://doi.org/10.1007/bf00331618
228 rdf:type schema:CreativeWork
229 sg:pub.10.1007/bf00338085 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032502861
230 https://doi.org/10.1007/bf00338085
231 rdf:type schema:CreativeWork
232 sg:pub.10.1007/bf00425708 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006156766
233 https://doi.org/10.1007/bf00425708
234 rdf:type schema:CreativeWork
235 sg:pub.10.1007/bf00752901 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025672137
236 https://doi.org/10.1007/bf00752901
237 rdf:type schema:CreativeWork
238 sg:pub.10.1007/bf02428025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009806071
239 https://doi.org/10.1007/bf02428025
240 rdf:type schema:CreativeWork
241 sg:pub.10.1007/bf02667740 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017993505
242 https://doi.org/10.1007/bf02667740
243 rdf:type schema:CreativeWork
244 sg:pub.10.1038/277129a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032852748
245 https://doi.org/10.1038/277129a0
246 rdf:type schema:CreativeWork
247 sg:pub.10.1038/292860a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024461091
248 https://doi.org/10.1038/292860a0
249 rdf:type schema:CreativeWork
250 sg:pub.10.1038/328203a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023828213
251 https://doi.org/10.1038/328203a0
252 rdf:type schema:CreativeWork
253 sg:pub.10.1038/328248a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052533155
254 https://doi.org/10.1038/328248a0
255 rdf:type schema:CreativeWork
256 sg:pub.10.1038/328251a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053741745
257 https://doi.org/10.1038/328251a0
258 rdf:type schema:CreativeWork
259 sg:pub.10.1038/348749a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038802363
260 https://doi.org/10.1038/348749a0
261 rdf:type schema:CreativeWork
262 sg:pub.10.1038/newbio244029a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022855491
263 https://doi.org/10.1038/newbio244029a0
264 rdf:type schema:CreativeWork
265 https://app.dimensions.ai/details/publication/pub.1077114457 schema:CreativeWork
266 https://app.dimensions.ai/details/publication/pub.1078652042 schema:CreativeWork
267 https://app.dimensions.ai/details/publication/pub.1079420313 schema:CreativeWork
268 https://app.dimensions.ai/details/publication/pub.1081614666 schema:CreativeWork
269 https://doi.org/10.1002/j.1460-2075.1983.tb01715.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077183620
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1002/j.1460-2075.1984.tb02202.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077183717
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1002/j.1460-2075.1985.tb03626.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077183746
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1002/j.1460-2075.1985.tb03949.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077060020
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1002/j.1460-2075.1985.tb04046.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077183792
278 rdf:type schema:CreativeWork
279 https://doi.org/10.1002/j.1460-2075.1987.tb02348.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077183914
280 rdf:type schema:CreativeWork
281 https://doi.org/10.1002/j.1460-2075.1987.tb04753.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1079517126
282 rdf:type schema:CreativeWork
283 https://doi.org/10.1002/j.1460-2075.1989.tb03383.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1079178806
284 rdf:type schema:CreativeWork
285 https://doi.org/10.1002/j.1460-2075.1989.tb03421.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077184027
286 rdf:type schema:CreativeWork
287 https://doi.org/10.1002/j.1460-2075.1989.tb03466.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1079008175
288 rdf:type schema:CreativeWork
289 https://doi.org/10.1016/0003-2697(76)90527-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025529346
290 rdf:type schema:CreativeWork
291 https://doi.org/10.1016/0092-8674(86)90762-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038072915
292 rdf:type schema:CreativeWork
293 https://doi.org/10.1016/0092-8674(87)90584-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041471577
294 rdf:type schema:CreativeWork
295 https://doi.org/10.1016/0168-9452(89)90121-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046326870
296 rdf:type schema:CreativeWork
297 https://doi.org/10.1016/s0022-2836(75)80083-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033296365
298 rdf:type schema:CreativeWork
299 https://doi.org/10.1016/s0044-328x(76)80093-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024944233
300 rdf:type schema:CreativeWork
301 https://doi.org/10.1016/s0065-2660(08)60040-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036596913
302 rdf:type schema:CreativeWork
303 https://doi.org/10.1073/pnas.82.19.6384 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040101303
304 rdf:type schema:CreativeWork
305 https://doi.org/10.1073/pnas.83.22.8447 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047987843
306 rdf:type schema:CreativeWork
307 https://doi.org/10.1073/pnas.83.6.1767 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022091054
308 rdf:type schema:CreativeWork
309 https://doi.org/10.1073/pnas.86.9.3219 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009821741
310 rdf:type schema:CreativeWork
311 https://doi.org/10.1089/dna.1986.5.101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059250789
312 rdf:type schema:CreativeWork
313 https://doi.org/10.1093/nar/12.5.2391 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040662176
314 rdf:type schema:CreativeWork
315 https://doi.org/10.1093/nar/13.19.6981 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006386102
316 rdf:type schema:CreativeWork
317 https://doi.org/10.1093/nar/15.24.10159 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018766354
318 rdf:type schema:CreativeWork
319 https://doi.org/10.1093/nar/16.15.7601 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019876717
320 rdf:type schema:CreativeWork
321 https://doi.org/10.1093/nar/16.19.9267 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059931705
322 rdf:type schema:CreativeWork
323 https://doi.org/10.1104/pp.81.1.86 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000555249
324 rdf:type schema:CreativeWork
325 https://doi.org/10.1105/tpc.1.1.141 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009036462
326 rdf:type schema:CreativeWork
327 https://doi.org/10.1105/tpc.2.4.279 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014921424
328 rdf:type schema:CreativeWork
329 https://doi.org/10.1105/tpc.2.4.291 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017136927
330 rdf:type schema:CreativeWork
331 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1023703429
332 rdf:type schema:CreativeWork
333 https://doi.org/10.1126/science.232.4754.1106 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062532481
334 rdf:type schema:CreativeWork
335 https://doi.org/10.2135/cropsci1978.0011183x001800060011x schema:sameAs https://app.dimensions.ai/details/publication/pub.1069018052
336 rdf:type schema:CreativeWork
337 https://doi.org/10.2307/3869070 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070467152
338 rdf:type schema:CreativeWork
339 https://doi.org/10.2307/3869076 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070467156
340 rdf:type schema:CreativeWork
341 https://doi.org/10.2307/3869077 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070467157
342 rdf:type schema:CreativeWork
343 https://www.grid.ac/institutes/grid.24805.3b schema:alternateName New Mexico State University
344 schema:name Chemistry Department, New Mexico State University, 88003-0001, Las Cruces, NM, USA
345 Graduate Program in Molecular Biology, New Mexico State University, 88003-0001, Las Cruces, NM, USA
346 Plant Genetic Engineering Laboratory, New Mexico State University, 88003-0001, Las Cruces, NM, USA
347 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...