Genotype-dependent whole plant regeneration from protoplasts of red clover (Trifolium pratense L.) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1989-11

AUTHORS

J. R. Myers, J. W. Grosser, N. L. Taylor, G. B. Collins

ABSTRACT

Protoplasts are useful for subcellular studies, in vitro selection, somatic hybridization and transformation. Whole plant regeneration from protoplasts is a prerequisite to producing altered crop plants using these methods. Whole plant regeneration was achieved from leaf- and suspension culture-derived protoplasts of T. pratense. Regeneration was most dependent upon identifying genotypes with genetic capacity to regenerate. Additional factors that were used to select genotypes, but which proved to be less important, were a high rate of cell growth in culture and a high plating efficiency of protoplasts. One genotype was identified which had a regeneration response equivalent to that of T. rubens and which regenerated from both leaf- and suspension culture-derived protoplasts. More... »

PAGES

113-127

References to SciGraph publications

  • 1980-11. Genetic variation in tissue cultures of red clover in THEORETICAL AND APPLIED GENETICS
  • 1984-12. Hybrids between Trifolium medium and Trifolium pratense in HEREDITAS
  • 1982-12. Somatic embryogenesis from mesophyll protoplasts of Trigonella corniculata (leguminosae) in PLANT CELL REPORTS
  • 1984-09. Plant regeneration from long-term suspension cultures of white clover in PLANTA
  • 1986-06. Regeneration of isolated mesophyll and cell suspension protoplasts to plants in Stylosanthes guianensis. A tropical forage legume in PLANT CELL REPORTS
  • 1983-06. Mesophyll protoplasts of fenugreek (Trigonella foenumgraecum): Isolation, culture and shoot regeneration in PLANT CELL REPORTS
  • 1984-06. Plant regeneration from suspension culture and mesophyll protoplasts of Medicago sativa L. in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1987-12. Somatic hybridization between birdsfoot trefoil (Lotus corniculatus L.) and L. conimbricensis Willd in THEORETICAL AND APPLIED GENETICS
  • 1984-11. Intra-varietal variation for in vitro plant regeneration in the genus Trifolium in EUPHYTICA
  • 1982-03. Interspecific hybridization of red clover (Trifolium pratense L.) with T. sarosiense Hazsl. Using in vitro embryo rescue in THEORETICAL AND APPLIED GENETICS
  • 1983-10. An assessment of the cultural capabilities of Trifolium repens L. (white clover) and Onobrychis viciifolia Scop. (sainfoin) mesophyll protoplasts in PLANT CELL REPORTS
  • 1983-04. Prolific plant regeneration from protoplast-derived tissues of Lotus corniculatus L. (birdsfoot trefoil) in PLANT CELL REPORTS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00035811

    DOI

    http://dx.doi.org/10.1007/bf00035811

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1033054759


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