Development of an embryogenic suspension culture of soybean (Glycine max Merrill.) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1988-01

AUTHORS

John J. Finer, Akitsu Nagasawa

ABSTRACT

A rapidly growing, maintainable, embryogenic suspension culture of Glycine max L. Merrill. has been generated. The culture consisted almost entirely of clumps of proliferating globular embryos with very little nonembryogenic tissues. The number and size of somatic embryo clumps were used to quantify growth of embryogenic tissues under various conditions. Initiation and proliferation of this embryogenic suspension culture were dependent on the inoculum, method of subculture, and composition of the subculture medium. Twenty to 50 mg of highly embryogenic, early-staged soybean tissue were inoculated into 35 ml of liquid culture medium containing 5 mg 1−1 2,4-D and either 15 mM glutamine or preferably 5 mM asparagine. Suspension cultures were subcultured at the same inoculum density every 4 weeks. The embryos matured and germinated following placement on solid media, resulting in consistent plant regeneration. More... »

PAGES

125-136

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00035754

DOI

http://dx.doi.org/10.1007/bf00035754

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1026863866


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