Segregation of transgenes in maize View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-01

AUTHORS

T. Michael Spencer, James V. O'Brien, William G. Start, Thomas R. Adams, William J. Gordon-Kamm, Peggy G. Lemaux

ABSTRACT

Progeny recovered from backcrossed transgenic maize tissue culture regenerants (R0) were analyzed to determine the segregation, expression, and stability of the introduced genes. Transgenic A188 x B73 R0 plants (regenerated from embryogenic suspension culture cells transformed by microprojectile bombardment; see [9]) were pollinated with nontransformed B73 pollen. Inheritance of a selectable marker gene, bar, and a nonselectable marker gene, uidA, was analyzed in progeny (R1) representing four independent transformation events. Activity of the bar gene product, phosphinothricin acetyltransferase (PAT), was assessed in plants comprising the four R1 populations. The number of R1 plants containing PAT activity per total number of R1 plants recovered for each population was 2/7, 19/34, 3/14 and 73/73. Molecular analysis confirmed the segregation of bar in three R1 populations and the lack of segregation in one R1 population. Cosegregation analysis indicated genetic linkage of bar and uidA in all four R1 populations. Analysis of numerous R2 plants derived from crossing transformed R1 plants with nontransformed inbreds revealed 1:1 segregation of PAT activity in three of four lines, including the line that failed to segregate in the R1 generation. Integrated copies of bar in one line appeared to be unstable or poorly transmitted. More... »

PAGES

201-210

References to SciGraph publications

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  • 1990-08. Genetically Engineered Fertile Indica-Rice Recovered from Protoplasts in NATURE BIOTECHNOLOGY
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  • 1990-05. Bialaphos selection of stable transformants from maize cell culture in THEORETICAL AND APPLIED GENETICS
  • 1991-01. Transformation of maize using microprojectile bombardment: An update and perspective in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • 1987-04. High meiotic stability of a foreign gene introduced into tobacco by Agrobacterium-mediated transformation in MOLECULAR GENETICS AND GENOMICS
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  • 1987-05. High-velocity microprojectiles for delivering nucleic acids into living cells in NATURE
  • Journal

    TITLE

    Plant Molecular Biology

    ISSUE

    2

    VOLUME

    18

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00034949

    DOI

    http://dx.doi.org/10.1007/bf00034949

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1033924827

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/1731983


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