Control of hyperhydricity of mango somatic embryos View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1995-08

AUTHORS

Mary-Joy Monsalud, Helena Mathews, Richard E. Litz, Dennis J. Gray

ABSTRACT

Hyperhydricity of immature somatic embryos has been a limiting factor for the development of highly embryogenic suspension cultures of many important mango cultivars. Reversion of hyperhydricity was achieved in two ways: 1) heart-stage somatic embryos (2–3 mm length) were partially dehydrated under controlled conditions at high relative humidity (RH) for 24–48 h and 2) the gelling agent (Gel-Gro) concentration of the plant growth medium was increased from 2.0 to 6.0 g l-1. Partially dehydrated immature somatic embryos were normal in appearance. Somatic embryos that were partially dehydrated germinated precociously when cultured on maturation medium. Although abscisic acid (ABA) did not reverse hyperhydricity of primary somatic embryos, ABA did stimulate the reversal of this abnormal pattern of development among secondary embryos. ABA (500 μM) inhibited precocious germination and permitted somatic embryo maturation. Partially dehydrated, immature somatic embryos (4–7 mm long) remained viable for up to 32 days in the absence of maturation medium under high RH. More... »

PAGES

195-206

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00034238

DOI

http://dx.doi.org/10.1007/bf00034238

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1031322365


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