In vitro transformation of petunia cells by an improved method of co-cultivation with A. tumefaciens strains View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1984-11

AUTHORS

Robert T. Fraley, Robert B. Horsch, Antonius Matzke, Mary-Dell Chilton, William S. Chilton, Patricia R. Sanders

ABSTRACT

A method (termed co-cultivation) for transforming plant cells in vitro with A. tumefaciens strains, which was originally developed by Marton et al. (1978) Nature 277: 129–131, has been modified by the incorporation of a novel feeder plate culture system and been extended to use with petunia protoplasts. Using efficient cell plating and selection conditions for phytohormone-independent growth, large numbers of independent transformed calli can be obtained efficiently (∼10-1) and in less than 3 weeks following protoplast isolation. Southern hybridization analysis has confirmed that the majority of the resulting in vitro transformants contain a single copy of full length T-DNA.The high efficiency of this procedure allows simple screening to identify plant cells transformed by Ti plasmids attenuated by deletion of internal T-DNA regions. Results are presented that demonstrate the co-cultivation method can be used in conjunction with short term assays for monitoring plant gene expression. More... »

PAGES

371-378

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00033384

DOI

http://dx.doi.org/10.1007/bf00033384

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011235063

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24310570


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Biology, Washington University, 63130, St. Louis, MO, U.S.A.", 
          "id": "http://www.grid.ac/institutes/grid.4367.6", 
          "name": [
            "Monsanto Corporate Research & Development Staff, Biological Sciences, 800 N. Lindbergh, 63167, St. Louis, MO, U.S.A.", 
            "Department of Biology, Washington University, 63130, St. Louis, MO, U.S.A."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fraley", 
        "givenName": "Robert T.", 
        "id": "sg:person.01241226547.40", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01241226547.40"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Monsanto Corporate Research & Development Staff, Biological Sciences, 800 N. Lindbergh, 63167, St. Louis, MO, U.S.A.", 
          "id": "http://www.grid.ac/institutes/grid.418554.9", 
          "name": [
            "Monsanto Corporate Research & Development Staff, Biological Sciences, 800 N. Lindbergh, 63167, St. Louis, MO, U.S.A."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Horsch", 
        "givenName": "Robert B.", 
        "id": "sg:person.0717466357.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0717466357.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut f\u00fcr Molecular Biologie, Ostesreichische Akademie der Wissenschaften, A-5020, Salzburg, Austria", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Department of Biology, Washington University, 63130, St. Louis, MO, U.S.A.", 
            "Institut f\u00fcr Molecular Biologie, Ostesreichische Akademie der Wissenschaften, A-5020, Salzburg, Austria"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Matzke", 
        "givenName": "Antonius", 
        "id": "sg:person.0604170460.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0604170460.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "CIBA-Geigy Biotechnology Facility, P.O. Box 12257, 27709, Research Triangle Park, NC, U.S.A.", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Department of Biology, Washington University, 63130, St. Louis, MO, U.S.A.", 
            "CIBA-Geigy Biotechnology Facility, P.O. Box 12257, 27709, Research Triangle Park, NC, U.S.A."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chilton", 
        "givenName": "Mary-Dell", 
        "id": "sg:person.01047011220.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047011220.52"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Botany, North Carolina State University, 27650, Raleigh, NC, U.S.A.", 
          "id": "http://www.grid.ac/institutes/grid.40803.3f", 
          "name": [
            "Department of Biology, Washington University, 63130, St. Louis, MO, U.S.A.", 
            "Department of Botany, North Carolina State University, 27650, Raleigh, NC, U.S.A."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chilton", 
        "givenName": "William S.", 
        "id": "sg:person.01142177672.37", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01142177672.37"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Monsanto Corporate Research & Development Staff, Biological Sciences, 800 N. Lindbergh, 63167, St. Louis, MO, U.S.A.", 
          "id": "http://www.grid.ac/institutes/grid.418554.9", 
          "name": [
            "Monsanto Corporate Research & Development Staff, Biological Sciences, 800 N. Lindbergh, 63167, St. Louis, MO, U.S.A."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sanders", 
        "givenName": "Patricia R.", 
        "id": "sg:person.0765601557.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0765601557.52"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/277129a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032852748", 
          "https://doi.org/10.1038/277129a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4684-4544-2_13", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001340357", 
          "https://doi.org/10.1007/978-1-4684-4544-2_13"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/304184a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030766221", 
          "https://doi.org/10.1038/304184a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/303209a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051577901", 
          "https://doi.org/10.1038/303209a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02831500", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006897738", 
          "https://doi.org/10.1007/bf02831500"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1984-11", 
    "datePublishedReg": "1984-11-01", 
    "description": "A method (termed co-cultivation) for transforming plant cells in vitro with A. tumefaciens strains, which was originally developed by Marton et al. (1978) Nature 277: 129\u2013131, has been modified by the incorporation of a novel feeder plate culture system and been extended to use with petunia protoplasts. Using efficient cell plating and selection conditions for phytohormone-independent growth, large numbers of independent transformed calli can be obtained efficiently (\u223c10-1) and in less than 3 weeks following protoplast isolation. Southern hybridization analysis has confirmed that the majority of the resulting in vitro transformants contain a single copy of full length T-DNA.The high efficiency of this procedure allows simple screening to identify plant cells transformed by Ti plasmids attenuated by deletion of internal T-DNA regions. Results are presented that demonstrate the co-cultivation method can be used in conjunction with short term assays for monitoring plant gene expression.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/bf00033384", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1101246", 
        "issn": [
          "0167-4412", 
          "1573-5028"
        ], 
        "name": "Plant Molecular Biology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "3"
      }
    ], 
    "keywords": [
      "plant cells", 
      "full-length T-DNA", 
      "plant gene expression", 
      "T-DNA region", 
      "phytohormone-independent growth", 
      "Southern hybridization analysis", 
      "A. tumefaciens strain", 
      "T-DNA", 
      "Petunia cells", 
      "co-cultivation method", 
      "A. tumefaciens", 
      "Ti plasmid", 
      "single copy", 
      "protoplast isolation", 
      "gene expression", 
      "hybridization analysis", 
      "petunia protoplasts", 
      "short-term assays", 
      "selection conditions", 
      "culture system", 
      "cell plating", 
      "cells", 
      "Marton et al", 
      "tumefaciens", 
      "protoplasts", 
      "transformants", 
      "simple screening", 
      "deletion", 
      "plasmid", 
      "callus", 
      "copies", 
      "expression", 
      "large number", 
      "assays", 
      "isolation", 
      "growth", 
      "strains", 
      "region", 
      "screening", 
      "incorporation", 
      "analysis", 
      "number", 
      "majority", 
      "conditions", 
      "transformation", 
      "high efficiency", 
      "results", 
      "conjunction", 
      "improved method", 
      "system", 
      "et al", 
      "method", 
      "efficiency", 
      "plating", 
      "weeks", 
      "al", 
      "procedure"
    ], 
    "name": "In vitro transformation of petunia cells by an improved method of co-cultivation with A. tumefaciens strains", 
    "pagination": "371-378", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1011235063"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00033384"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "24310570"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00033384", 
      "https://app.dimensions.ai/details/publication/pub.1011235063"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-12-01T06:18", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/article/article_172.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/bf00033384"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00033384'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00033384'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00033384'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00033384'


 

This table displays all metadata directly associated to this object as RDF triples.

187 TRIPLES      21 PREDICATES      88 URIs      75 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00033384 schema:about anzsrc-for:06
2 anzsrc-for:0604
3 schema:author N8cdf514282ae4bbcad29a7d845f63fdc
4 schema:citation sg:pub.10.1007/978-1-4684-4544-2_13
5 sg:pub.10.1007/bf02831500
6 sg:pub.10.1038/277129a0
7 sg:pub.10.1038/303209a0
8 sg:pub.10.1038/304184a0
9 schema:datePublished 1984-11
10 schema:datePublishedReg 1984-11-01
11 schema:description A method (termed co-cultivation) for transforming plant cells in vitro with A. tumefaciens strains, which was originally developed by Marton et al. (1978) Nature 277: 129–131, has been modified by the incorporation of a novel feeder plate culture system and been extended to use with petunia protoplasts. Using efficient cell plating and selection conditions for phytohormone-independent growth, large numbers of independent transformed calli can be obtained efficiently (∼10-1) and in less than 3 weeks following protoplast isolation. Southern hybridization analysis has confirmed that the majority of the resulting in vitro transformants contain a single copy of full length T-DNA.The high efficiency of this procedure allows simple screening to identify plant cells transformed by Ti plasmids attenuated by deletion of internal T-DNA regions. Results are presented that demonstrate the co-cultivation method can be used in conjunction with short term assays for monitoring plant gene expression.
12 schema:genre article
13 schema:isAccessibleForFree false
14 schema:isPartOf Na81851e4289745c888414a7d765d6927
15 Nc59f1f00deda4782bac88c967f587cd7
16 sg:journal.1101246
17 schema:keywords A. tumefaciens
18 A. tumefaciens strain
19 Marton et al
20 Petunia cells
21 Southern hybridization analysis
22 T-DNA
23 T-DNA region
24 Ti plasmid
25 al
26 analysis
27 assays
28 callus
29 cell plating
30 cells
31 co-cultivation method
32 conditions
33 conjunction
34 copies
35 culture system
36 deletion
37 efficiency
38 et al
39 expression
40 full-length T-DNA
41 gene expression
42 growth
43 high efficiency
44 hybridization analysis
45 improved method
46 incorporation
47 isolation
48 large number
49 majority
50 method
51 number
52 petunia protoplasts
53 phytohormone-independent growth
54 plant cells
55 plant gene expression
56 plasmid
57 plating
58 procedure
59 protoplast isolation
60 protoplasts
61 region
62 results
63 screening
64 selection conditions
65 short-term assays
66 simple screening
67 single copy
68 strains
69 system
70 transformants
71 transformation
72 tumefaciens
73 weeks
74 schema:name In vitro transformation of petunia cells by an improved method of co-cultivation with A. tumefaciens strains
75 schema:pagination 371-378
76 schema:productId N508ed009717b4eb48264bc373c91d2b4
77 N8aed827904254f489b8f58dfce37ad27
78 Nf543b2e18d784ef2b5d4d3d2010daa76
79 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011235063
80 https://doi.org/10.1007/bf00033384
81 schema:sdDatePublished 2022-12-01T06:18
82 schema:sdLicense https://scigraph.springernature.com/explorer/license/
83 schema:sdPublisher Ncd947b5502f5483781898efa67130e8f
84 schema:url https://doi.org/10.1007/bf00033384
85 sgo:license sg:explorer/license/
86 sgo:sdDataset articles
87 rdf:type schema:ScholarlyArticle
88 N29e2c4d5c0604a9fb474d9f5ac318e4d rdf:first sg:person.01047011220.52
89 rdf:rest Nbf8f8081a1ef48d39d45455df6d2ffec
90 N508ed009717b4eb48264bc373c91d2b4 schema:name dimensions_id
91 schema:value pub.1011235063
92 rdf:type schema:PropertyValue
93 N68b6a5287dc34d80bddddaed299018f5 rdf:first sg:person.0717466357.15
94 rdf:rest N8ed03b3cc01447518805e02d93a342f4
95 N8aed827904254f489b8f58dfce37ad27 schema:name doi
96 schema:value 10.1007/bf00033384
97 rdf:type schema:PropertyValue
98 N8cdf514282ae4bbcad29a7d845f63fdc rdf:first sg:person.01241226547.40
99 rdf:rest N68b6a5287dc34d80bddddaed299018f5
100 N8ed03b3cc01447518805e02d93a342f4 rdf:first sg:person.0604170460.05
101 rdf:rest N29e2c4d5c0604a9fb474d9f5ac318e4d
102 N9ba241de6fb146fdbceeb4dead374743 rdf:first sg:person.0765601557.52
103 rdf:rest rdf:nil
104 Na81851e4289745c888414a7d765d6927 schema:issueNumber 6
105 rdf:type schema:PublicationIssue
106 Nbf8f8081a1ef48d39d45455df6d2ffec rdf:first sg:person.01142177672.37
107 rdf:rest N9ba241de6fb146fdbceeb4dead374743
108 Nc59f1f00deda4782bac88c967f587cd7 schema:volumeNumber 3
109 rdf:type schema:PublicationVolume
110 Ncd947b5502f5483781898efa67130e8f schema:name Springer Nature - SN SciGraph project
111 rdf:type schema:Organization
112 Nf543b2e18d784ef2b5d4d3d2010daa76 schema:name pubmed_id
113 schema:value 24310570
114 rdf:type schema:PropertyValue
115 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
116 schema:name Biological Sciences
117 rdf:type schema:DefinedTerm
118 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
119 schema:name Genetics
120 rdf:type schema:DefinedTerm
121 sg:journal.1101246 schema:issn 0167-4412
122 1573-5028
123 schema:name Plant Molecular Biology
124 schema:publisher Springer Nature
125 rdf:type schema:Periodical
126 sg:person.01047011220.52 schema:affiliation grid-institutes:None
127 schema:familyName Chilton
128 schema:givenName Mary-Dell
129 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047011220.52
130 rdf:type schema:Person
131 sg:person.01142177672.37 schema:affiliation grid-institutes:grid.40803.3f
132 schema:familyName Chilton
133 schema:givenName William S.
134 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01142177672.37
135 rdf:type schema:Person
136 sg:person.01241226547.40 schema:affiliation grid-institutes:grid.4367.6
137 schema:familyName Fraley
138 schema:givenName Robert T.
139 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01241226547.40
140 rdf:type schema:Person
141 sg:person.0604170460.05 schema:affiliation grid-institutes:None
142 schema:familyName Matzke
143 schema:givenName Antonius
144 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0604170460.05
145 rdf:type schema:Person
146 sg:person.0717466357.15 schema:affiliation grid-institutes:grid.418554.9
147 schema:familyName Horsch
148 schema:givenName Robert B.
149 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0717466357.15
150 rdf:type schema:Person
151 sg:person.0765601557.52 schema:affiliation grid-institutes:grid.418554.9
152 schema:familyName Sanders
153 schema:givenName Patricia R.
154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0765601557.52
155 rdf:type schema:Person
156 sg:pub.10.1007/978-1-4684-4544-2_13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001340357
157 https://doi.org/10.1007/978-1-4684-4544-2_13
158 rdf:type schema:CreativeWork
159 sg:pub.10.1007/bf02831500 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006897738
160 https://doi.org/10.1007/bf02831500
161 rdf:type schema:CreativeWork
162 sg:pub.10.1038/277129a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032852748
163 https://doi.org/10.1038/277129a0
164 rdf:type schema:CreativeWork
165 sg:pub.10.1038/303209a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051577901
166 https://doi.org/10.1038/303209a0
167 rdf:type schema:CreativeWork
168 sg:pub.10.1038/304184a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030766221
169 https://doi.org/10.1038/304184a0
170 rdf:type schema:CreativeWork
171 grid-institutes:None schema:alternateName CIBA-Geigy Biotechnology Facility, P.O. Box 12257, 27709, Research Triangle Park, NC, U.S.A.
172 Institut für Molecular Biologie, Ostesreichische Akademie der Wissenschaften, A-5020, Salzburg, Austria
173 schema:name CIBA-Geigy Biotechnology Facility, P.O. Box 12257, 27709, Research Triangle Park, NC, U.S.A.
174 Department of Biology, Washington University, 63130, St. Louis, MO, U.S.A.
175 Institut für Molecular Biologie, Ostesreichische Akademie der Wissenschaften, A-5020, Salzburg, Austria
176 rdf:type schema:Organization
177 grid-institutes:grid.40803.3f schema:alternateName Department of Botany, North Carolina State University, 27650, Raleigh, NC, U.S.A.
178 schema:name Department of Biology, Washington University, 63130, St. Louis, MO, U.S.A.
179 Department of Botany, North Carolina State University, 27650, Raleigh, NC, U.S.A.
180 rdf:type schema:Organization
181 grid-institutes:grid.418554.9 schema:alternateName Monsanto Corporate Research & Development Staff, Biological Sciences, 800 N. Lindbergh, 63167, St. Louis, MO, U.S.A.
182 schema:name Monsanto Corporate Research & Development Staff, Biological Sciences, 800 N. Lindbergh, 63167, St. Louis, MO, U.S.A.
183 rdf:type schema:Organization
184 grid-institutes:grid.4367.6 schema:alternateName Department of Biology, Washington University, 63130, St. Louis, MO, U.S.A.
185 schema:name Department of Biology, Washington University, 63130, St. Louis, MO, U.S.A.
186 Monsanto Corporate Research & Development Staff, Biological Sciences, 800 N. Lindbergh, 63167, St. Louis, MO, U.S.A.
187 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...