Transfer of non-T-DNA portions of the Agrobacterium tumefaciens Ti plasmid pTiA6 from the left terminus of TL-DNA View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1995-09

AUTHORS

Vai. Ramanathan, K. Veluthambi

ABSTRACT

We introduced a plant selection marker, nptII, to the left of border A in the Agrobacterium Ti plasmid pTiA6. Infection of tobacco leaf discs with the modified Agrobacterium strain gave rise to kanamycin-resistant calli which grew in a hormone-dependent manner. Southern hybridization analysis of DNA isolated from four transformants indicated initiation of DNA transfer at or near border A and absence of T-DNA sequences. These results demonstrate that DNA transfer events starting at a left border on a native Ti plasmid and moving away from the T-DNA region occur and that they can be detected by designing a suitable selection strategy. More... »

PAGES

1149-1154

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00032676

DOI

http://dx.doi.org/10.1007/bf00032676

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1021795493

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/7548833


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