A set of novel Ti plasmid-derived vectors for the production of transgenic plants View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1986-09

AUTHORS

A. J. M. Matzke, M. A. Matzke

ABSTRACT

We describe in this paper the construction and use of a set of novel Ti plasmid-derived vectors that can be used to produce transgenic plants. These vectors are based on one of two strategies: 1) double recombination into the wild-type Ti plasmid of genetic information flanked by two T-DNA fragments on a wide-host range plasmid; 2) the binary vector strategy. The vector based on the double recombination principle contains a kanamycin resistance gene for use as a plant selectable marker, a polylinker for the insertion of foreign genes, and a nopaline synthase gene. The vector was constructed such that a disarmed T-DNA results from the double recombination event. The binary vector combines several advantageous features including an origin of replication that is stable in Agrobacterium in the absence of selection, six unique sites for insertion of foreign genes, an intact nopaline synthase gene, and a kanamycin resistance marker for selection of transformed plant cells. All of these vectors have been used to produce tobacco plants transformed with a variety of foreign genes. More... »

PAGES

357-365

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00032565

DOI

http://dx.doi.org/10.1007/bf00032565

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1039046390

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24302405


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Plant Biology", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Institute of Molecular Biology, Austrian Academy of Sciences, Billrothstrasse 11, A-5020, Salzburg, Austria", 
          "id": "http://www.grid.ac/institutes/grid.4299.6", 
          "name": [
            "Institute of Molecular Biology, Austrian Academy of Sciences, Billrothstrasse 11, A-5020, Salzburg, Austria"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Matzke", 
        "givenName": "A. J. M.", 
        "id": "sg:person.012521372244.49", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012521372244.49"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Molecular Biology, Austrian Academy of Sciences, Billrothstrasse 11, A-5020, Salzburg, Austria", 
          "id": "http://www.grid.ac/institutes/grid.4299.6", 
          "name": [
            "Institute of Molecular Biology, Austrian Academy of Sciences, Billrothstrasse 11, A-5020, Salzburg, Austria"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Matzke", 
        "givenName": "M. A.", 
        "id": "sg:person.01366677656.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01366677656.47"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/978-3-642-68315-2_4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1081656920", 
          "https://doi.org/10.1007/978-3-642-68315-2_4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0785-637", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037136744", 
          "https://doi.org/10.1038/nbt0785-637"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00015678", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022322014", 
          "https://doi.org/10.1007/bf00015678"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00020090", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032948994", 
          "https://doi.org/10.1007/bf00020090"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00020092", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040103117", 
          "https://doi.org/10.1007/bf00020092"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/304184a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030766221", 
          "https://doi.org/10.1038/304184a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0583-262", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029030862", 
          "https://doi.org/10.1038/nbt0583-262"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00327434", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008433052", 
          "https://doi.org/10.1007/bf00327434"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0785-629", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029486253", 
          "https://doi.org/10.1038/nbt0785-629"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02885590", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036411435", 
          "https://doi.org/10.1007/bf02885590"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/303179a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018421874", 
          "https://doi.org/10.1038/303179a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00034942", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002812691", 
          "https://doi.org/10.1007/bf00034942"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00033384", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011235063", 
          "https://doi.org/10.1007/bf00033384"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1986-09", 
    "datePublishedReg": "1986-09-01", 
    "description": "We describe in this paper the construction and use of a set of novel Ti plasmid-derived vectors that can be used to produce transgenic plants. These vectors are based on one of two strategies: 1) double recombination into the wild-type Ti plasmid of genetic information flanked by two T-DNA fragments on a wide-host range plasmid; 2) the binary vector strategy. The vector based on the double recombination principle contains a kanamycin resistance gene for use as a plant selectable marker, a polylinker for the insertion of foreign genes, and a nopaline synthase gene. The vector was constructed such that a disarmed T-DNA results from the double recombination event. The binary vector combines several advantageous features including an origin of replication that is stable in Agrobacterium in the absence of selection, six unique sites for insertion of foreign genes, an intact nopaline synthase gene, and a kanamycin resistance marker for selection of transformed plant cells. All of these vectors have been used to produce tobacco plants transformed with a variety of foreign genes.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/bf00032565", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1101246", 
        "issn": [
          "0167-4412", 
          "1573-5028"
        ], 
        "name": "Plant Molecular Biology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "7"
      }
    ], 
    "keywords": [
      "Ti plasmid-derived vectors", 
      "nopaline synthase gene", 
      "foreign genes", 
      "transgenic plants", 
      "synthase gene", 
      "wild-type Ti plasmid", 
      "binary vector strategy", 
      "T-DNA fragment", 
      "plant selectable marker", 
      "wide host range plasmid", 
      "origin of replication", 
      "T-DNA results", 
      "absence of selection", 
      "kanamycin resistance gene", 
      "double recombination event", 
      "kanamycin resistance marker", 
      "plant cells", 
      "tobacco plants", 
      "Ti plasmid", 
      "selectable marker", 
      "recombination events", 
      "genetic information", 
      "resistance genes", 
      "genes", 
      "double recombination", 
      "binary vector", 
      "range plasmid", 
      "plants", 
      "unique site", 
      "resistance markers", 
      "plasmid", 
      "Agrobacterium", 
      "vector strategy", 
      "polylinker", 
      "markers", 
      "vector", 
      "recombination", 
      "replication", 
      "insertion", 
      "selection", 
      "cells", 
      "fragments", 
      "sites", 
      "production", 
      "origin", 
      "absence", 
      "variety", 
      "strategies", 
      "events", 
      "set", 
      "advantageous features", 
      "features", 
      "use", 
      "information", 
      "results", 
      "construction", 
      "principles", 
      "paper"
    ], 
    "name": "A set of novel Ti plasmid-derived vectors for the production of transgenic plants", 
    "pagination": "357-365", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1039046390"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00032565"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "24302405"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00032565", 
      "https://app.dimensions.ai/details/publication/pub.1039046390"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-12-01T06:19", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/article/article_198.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/bf00032565"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00032565'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00032565'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00032565'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00032565'


 

This table displays all metadata directly associated to this object as RDF triples.

182 TRIPLES      21 PREDICATES      98 URIs      76 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00032565 schema:about anzsrc-for:06
2 anzsrc-for:0604
3 anzsrc-for:0607
4 schema:author Nfff8e8616d1f4adc87951e796502d189
5 schema:citation sg:pub.10.1007/978-3-642-68315-2_4
6 sg:pub.10.1007/bf00015678
7 sg:pub.10.1007/bf00020090
8 sg:pub.10.1007/bf00020092
9 sg:pub.10.1007/bf00033384
10 sg:pub.10.1007/bf00034942
11 sg:pub.10.1007/bf00327434
12 sg:pub.10.1007/bf02885590
13 sg:pub.10.1038/303179a0
14 sg:pub.10.1038/304184a0
15 sg:pub.10.1038/nbt0583-262
16 sg:pub.10.1038/nbt0785-629
17 sg:pub.10.1038/nbt0785-637
18 schema:datePublished 1986-09
19 schema:datePublishedReg 1986-09-01
20 schema:description We describe in this paper the construction and use of a set of novel Ti plasmid-derived vectors that can be used to produce transgenic plants. These vectors are based on one of two strategies: 1) double recombination into the wild-type Ti plasmid of genetic information flanked by two T-DNA fragments on a wide-host range plasmid; 2) the binary vector strategy. The vector based on the double recombination principle contains a kanamycin resistance gene for use as a plant selectable marker, a polylinker for the insertion of foreign genes, and a nopaline synthase gene. The vector was constructed such that a disarmed T-DNA results from the double recombination event. The binary vector combines several advantageous features including an origin of replication that is stable in Agrobacterium in the absence of selection, six unique sites for insertion of foreign genes, an intact nopaline synthase gene, and a kanamycin resistance marker for selection of transformed plant cells. All of these vectors have been used to produce tobacco plants transformed with a variety of foreign genes.
21 schema:genre article
22 schema:isAccessibleForFree false
23 schema:isPartOf Na3ec63ce39b24086a23c0a7dd0224dec
24 Ne02925b035434827b0b528177ec97fbb
25 sg:journal.1101246
26 schema:keywords Agrobacterium
27 T-DNA fragment
28 T-DNA results
29 Ti plasmid
30 Ti plasmid-derived vectors
31 absence
32 absence of selection
33 advantageous features
34 binary vector
35 binary vector strategy
36 cells
37 construction
38 double recombination
39 double recombination event
40 events
41 features
42 foreign genes
43 fragments
44 genes
45 genetic information
46 information
47 insertion
48 kanamycin resistance gene
49 kanamycin resistance marker
50 markers
51 nopaline synthase gene
52 origin
53 origin of replication
54 paper
55 plant cells
56 plant selectable marker
57 plants
58 plasmid
59 polylinker
60 principles
61 production
62 range plasmid
63 recombination
64 recombination events
65 replication
66 resistance genes
67 resistance markers
68 results
69 selectable marker
70 selection
71 set
72 sites
73 strategies
74 synthase gene
75 tobacco plants
76 transgenic plants
77 unique site
78 use
79 variety
80 vector
81 vector strategy
82 wide host range plasmid
83 wild-type Ti plasmid
84 schema:name A set of novel Ti plasmid-derived vectors for the production of transgenic plants
85 schema:pagination 357-365
86 schema:productId N63a4b4461579424e8cecd12ff3f2f0f5
87 N7286ee6d3f0541f7b7aeb09b4d6cfab1
88 N78095566863d43a592ddfa51fefd1d55
89 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039046390
90 https://doi.org/10.1007/bf00032565
91 schema:sdDatePublished 2022-12-01T06:19
92 schema:sdLicense https://scigraph.springernature.com/explorer/license/
93 schema:sdPublisher N963753d5f07b47aca25cd6c83b3c4a8c
94 schema:url https://doi.org/10.1007/bf00032565
95 sgo:license sg:explorer/license/
96 sgo:sdDataset articles
97 rdf:type schema:ScholarlyArticle
98 N63a4b4461579424e8cecd12ff3f2f0f5 schema:name doi
99 schema:value 10.1007/bf00032565
100 rdf:type schema:PropertyValue
101 N7286ee6d3f0541f7b7aeb09b4d6cfab1 schema:name dimensions_id
102 schema:value pub.1039046390
103 rdf:type schema:PropertyValue
104 N78095566863d43a592ddfa51fefd1d55 schema:name pubmed_id
105 schema:value 24302405
106 rdf:type schema:PropertyValue
107 N963753d5f07b47aca25cd6c83b3c4a8c schema:name Springer Nature - SN SciGraph project
108 rdf:type schema:Organization
109 Na3ec63ce39b24086a23c0a7dd0224dec schema:issueNumber 5
110 rdf:type schema:PublicationIssue
111 Nbd43164f9b7946b39b7259a1d168498b rdf:first sg:person.01366677656.47
112 rdf:rest rdf:nil
113 Ne02925b035434827b0b528177ec97fbb schema:volumeNumber 7
114 rdf:type schema:PublicationVolume
115 Nfff8e8616d1f4adc87951e796502d189 rdf:first sg:person.012521372244.49
116 rdf:rest Nbd43164f9b7946b39b7259a1d168498b
117 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
118 schema:name Biological Sciences
119 rdf:type schema:DefinedTerm
120 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
121 schema:name Genetics
122 rdf:type schema:DefinedTerm
123 anzsrc-for:0607 schema:inDefinedTermSet anzsrc-for:
124 schema:name Plant Biology
125 rdf:type schema:DefinedTerm
126 sg:journal.1101246 schema:issn 0167-4412
127 1573-5028
128 schema:name Plant Molecular Biology
129 schema:publisher Springer Nature
130 rdf:type schema:Periodical
131 sg:person.012521372244.49 schema:affiliation grid-institutes:grid.4299.6
132 schema:familyName Matzke
133 schema:givenName A. J. M.
134 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012521372244.49
135 rdf:type schema:Person
136 sg:person.01366677656.47 schema:affiliation grid-institutes:grid.4299.6
137 schema:familyName Matzke
138 schema:givenName M. A.
139 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01366677656.47
140 rdf:type schema:Person
141 sg:pub.10.1007/978-3-642-68315-2_4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1081656920
142 https://doi.org/10.1007/978-3-642-68315-2_4
143 rdf:type schema:CreativeWork
144 sg:pub.10.1007/bf00015678 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022322014
145 https://doi.org/10.1007/bf00015678
146 rdf:type schema:CreativeWork
147 sg:pub.10.1007/bf00020090 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032948994
148 https://doi.org/10.1007/bf00020090
149 rdf:type schema:CreativeWork
150 sg:pub.10.1007/bf00020092 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040103117
151 https://doi.org/10.1007/bf00020092
152 rdf:type schema:CreativeWork
153 sg:pub.10.1007/bf00033384 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011235063
154 https://doi.org/10.1007/bf00033384
155 rdf:type schema:CreativeWork
156 sg:pub.10.1007/bf00034942 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002812691
157 https://doi.org/10.1007/bf00034942
158 rdf:type schema:CreativeWork
159 sg:pub.10.1007/bf00327434 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008433052
160 https://doi.org/10.1007/bf00327434
161 rdf:type schema:CreativeWork
162 sg:pub.10.1007/bf02885590 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036411435
163 https://doi.org/10.1007/bf02885590
164 rdf:type schema:CreativeWork
165 sg:pub.10.1038/303179a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018421874
166 https://doi.org/10.1038/303179a0
167 rdf:type schema:CreativeWork
168 sg:pub.10.1038/304184a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030766221
169 https://doi.org/10.1038/304184a0
170 rdf:type schema:CreativeWork
171 sg:pub.10.1038/nbt0583-262 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029030862
172 https://doi.org/10.1038/nbt0583-262
173 rdf:type schema:CreativeWork
174 sg:pub.10.1038/nbt0785-629 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029486253
175 https://doi.org/10.1038/nbt0785-629
176 rdf:type schema:CreativeWork
177 sg:pub.10.1038/nbt0785-637 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037136744
178 https://doi.org/10.1038/nbt0785-637
179 rdf:type schema:CreativeWork
180 grid-institutes:grid.4299.6 schema:alternateName Institute of Molecular Biology, Austrian Academy of Sciences, Billrothstrasse 11, A-5020, Salzburg, Austria
181 schema:name Institute of Molecular Biology, Austrian Academy of Sciences, Billrothstrasse 11, A-5020, Salzburg, Austria
182 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...