A chimeric transcript containing a 16 S rRNA and a potential mRNA in chloroplasts of Euglena gracilis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1988-07

AUTHORS

Barbara Koller, Etienne Roux, Paul-Etienne Montandon, Erhard Stutz

ABSTRACT

The chloroplast genome of Euglena gracilis contains a supplementary gene for a 16 S rRNA (s16 S rrn gene), which is not part of a complete rrn operon. An open reading frame (ORF406) is located downstream of the s16 S rrn gene. Chloroplast RNA was hybridized with cloned DNA fragments of this region and the hybrids were analysed by electron microscopy and S1-nuclease protection experiments. The s16 S rrn gene and the ORF406 are transcribed as one continuous 3.6 kb long RNA, which starts just upstream of the 5'-end of the s16 S rrn gene. The 3'-end occurs at multiple sites within a region of 700 bases downstream of the ORF. Northern blot analysis shows that the abundance of the transcript is comparable with that of other chloroplast mRNAs. More... »

PAGES

339-347

References to SciGraph publications

Journal

TITLE

Plant Molecular Biology

ISSUE

4

VOLUME

10

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00029884

DOI

http://dx.doi.org/10.1007/bf00029884

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1028479118

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24277565


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