Production of the new antimalarial drug artemisinin in shoot cultures of Artemisia annua L. View Full Text


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Article Info

DATE

1993-02

AUTHORS

Herman J. Woerdenbag, Jos F. J. Lüers, Wim van Uden, Niesko Pras, Theo M. Malingré, A. Wilhelm Alfermann

ABSTRACT

From aseptically grown Artemisia annua plantlets, shoot cultures were initiated. Using different concentrations of auxine, cytokinine and sucrose, a suitable culture medium was developed, with respect to the growth of the shoots and their artemisinin accumulation. Nitrate concentration and conductivity appeared to be suitable growth parameters. The artemisinin content was measured gas chromatographically. The shoot cultures were maintained in the developed standard medium, consisting of a half concentration of MS-salts with vitamins, 0.2 mg l-1 BAP, 0.05 mg l-1 NAA and 1% sucrose. The growth of the shoots and the artemisinin content remained stable for a longer period. They showed considerable photosynthetic activity and generally contained ca. 0.08% artemisinin on a dry weight basis. The highest artemisinin content found was 0.16% in the above mentioned standard medium, but also on the same medium with 0.5% sucrose. Attempts were made to further improve the artemisinin production by varying the medium composition through addition of gibberellic acid or casein hydroly-state; by omitting plant growth regulators; by precursor feeding, i.e. mevalonic acid; by influencing the biosynthesis routing through inhibition of the sterol synthesis by miconazole, naftifine or terbinafine; by changing gene expression with 5-azacytidine or colchicine; and by elicitation, using cellulase, chitosan, glutathione or nigeran. Enhanced artemisinin production was found with 10 mg l-1 gibberellic acid, 0.5 g l-1 casein hydrolysate, 10 mg l-1 or 20 mg l-1 naftifine. Relative increases of 154%, 169%, 140% and 120% were found, respectively. Other additions caused the growth to cease and the artemisinin contents to drop. More... »

PAGES

247-257

References to SciGraph publications

  • 1989-10. Antiplasmodial activity of Artemisia annua plant cell cultures in PLANT CELL REPORTS
  • 1990-05. Tissue cultures of Artemisia pallens: Organogenesis, terpenoid production in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1984-09. Influence of colchicine treatment on chromosome number and growth rate of tissue cultures of Valeriana wallichii DC in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1990-10. Artemisia annua L.: a source of novel antimalarial drugs in INTERNATIONAL JOURNAL OF CLINICAL PHARMACY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00029850

    DOI

    http://dx.doi.org/10.1007/bf00029850

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1012709986


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