Promoter methylation and progressive transgene inactivation inArabidopsis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-10

AUTHORS

Nigel J. Kilby, H. M. Ottoline Leyser, Ian J. Furner

ABSTRACT

Agrobacterium-transformedArabidopsis plants were generated and the stability of their T-DNA-encoded resistance to kanamycin was examined. Of seven families, each homozygous for a single insertion event, two showed progressive inactivation of resistance over four generations of inbreeding. Loss of resistance was associated with methylation of anSst II site in thenos promoter of the kanamycin resistance gene. Treatment of plant roots from inactive lines with the demethylating agent 5-azacytidine restored the ability of such lines to form callus on kanamycin-containing media. These observations are consistent with the view that methylation is a factor in the progressive inactivation of transgenes inArabidopsis. More... »

PAGES

103-112

References to SciGraph publications

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  • 1991-07. Transgene expression variability (position effect) of CAT and GUS reporter genes driven by linked divergent T-DNA promoters in PLANT MOLECULAR BIOLOGY
  • 1991-07. 5-Azacytidine-induced hypomethylation of tobacco HRS60 tandem DNA repeats in tissue culture in PLANTA
  • 1990-12. The effect of T-DNA copy number, position and methylation on reporter gene expression in tobacco transformants in PLANT MOLECULAR BIOLOGY
  • 1987-05. T-DNA structure and gene expression in petunia plants transformed by Agrobacterium tumefaciens C58 derivatives in MOLECULAR GENETICS AND GENOMICS
  • 1984-11. Silent T-DNA genes in plant lines transformed by Agrobacterium tumefaciens are activated by grafting and by 5-azacytidine treatment in PLANT MOLECULAR BIOLOGY
  • 1990-07. Epigenetic changes in the expression of the maize A1 gene inPetunia hybrida: Role of numbers of integrated gene copies and state of methylation in MOLECULAR GENETICS AND GENOMICS
  • 1991-05. Differential inactivation and methylation of a transgene in plants by two suppressor loci containing homologous sequences in PLANT MOLECULAR BIOLOGY
  • 1988-12. Genetic analysis of T-DNA insertions into the tobacco genome in PLANT CELL REPORTS
  • 1988-12. High efficiency Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana leaf and cotyledon explants in PLANT CELL REPORTS
  • 1986-07. Clones from a shooty tobacco crown gall tumor II: irregular T-DNA structures and organization, T-DNA methylation and conditional expression of opine genes in PLANT MOLECULAR BIOLOGY
  • 1987. Direct Gene Transfer to Plants in PLANT DNA INFECTIOUS AGENTS
  • 1981-08. Sequence specificity of methylation in higher plant DNA in NATURE
  • 1986-05. Both TATA box and upstream regions are required for the nopaline synthase promoter activity in transformed tobacco cells in MOLECULAR GENETICS AND GENOMICS
  • 1984-11. Changes in T-DNA methylation and expression are associated with phenotypic variation and plant regeneration in a crown gall tumor line in MOLECULAR GENETICS AND GENOMICS
  • 1988-05. Expression and inheritance of kanamycin resistance in a large number of transgenic petunias generated by Agrobacterium-mediated transformation in PLANT MOLECULAR BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00029153

    DOI

    http://dx.doi.org/10.1007/bf00029153

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1031933032

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/1325204


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