A cdc2 gene of Petunia hybrida is differentially expressed in leaves, protoplasts and during various cell cycle phases View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-12

AUTHORS

Catherine Bergounioux, Claudette Perennes, Adriana S. Hemerly, Li Xian Qin, Claudine Sarda, Dirk Inze, Pierre Gadal

ABSTRACT

Analysis of p34cdc2 kinase in higher eukaryotes has demonstrated that p34cdc2 function is conserved in all eukaryotic cells. The p34cdc2 kinase (the product of the cdc2 gene) is required during the G1 cell cycle phase at the initiation of DNA replication and also in G2-M phases for entry into mitosis. In this paper we report the isolation and characterization of a cdc2 Petunia hybrida PCR fragment (cdc2Pet). Using a DNA probe based on this fragment and a p34cdc2-specific antibody, cdc2Pet transcript and p34 protein levels were found to be constant both in 2C nuclei of highly proliferating mesophyll 2C cells derived from protoplasts and in 2C nuclei isolated directly from expanded petunia leaves. Both the cdc2Pet transcript and p34cdc2 protein levels were found to be higher in nuclei at 4C than in those at 2C, even when these 4C nuclei were from non-proliferating tissue. Thus cdc2Pet mRNA and protein levels measured in different tissues should not be interpreted to reflect exclusively the proliferative state of the tissue but also the frequency of G2 cells including those in the differentiated state. More... »

PAGES

1121-1130

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00028898

DOI

http://dx.doi.org/10.1007/bf00028898

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1031393822

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/1361156


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