Termini and telomeres in T-DNA transformation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1994-11

AUTHORS

Maurizio Chiurazzi, Ethan R. Signer

ABSTRACT

A T-DNA vector for plant transformation has been constructed in which the cloning site is located 9 bp from the right-border (RB) end and 27 bp from the left-border (LB) end. In this vector cloned DNA homologous to plant chromosomal sequences is located at the T-DNA termini, and will thus be exposed by even limited exonucleolysis in planta. The arabidopsis ADH (alcohol dehydrogenase) locus was mobilized from Agrobacterium, and integration into the recipient genome was studied. Despite the terminal location of ADH homology in this vector, the T-DNA integrated essentially at random in the Arabidopsis genome rather than at the endogenous ADH locus. T-DNA integration was blocked, however, when Arabidopsis telomeric sequences were added to the construct at each end of the ADH homology. Thus the predominant mode by which incoming T-DNA is integrated into the continuity of chromosomal DNA involves free DNA ends, but, in contrast to modes of recombination such as gap repair, does not involve extensive terminal DNA sequence homology. More... »

PAGES

923-934

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00028859

    DOI

    http://dx.doi.org/10.1007/bf00028859

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1035319839

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/8000005


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    RDF/XML is a standard XML format for linked data.

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