Treatment of Agrobacterium or leaf disks with 5-azacytidine increases transgene expression in tobacco View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-02

AUTHORS

Gorm Palmgren, Ole Mattson, Finn Thyge Okkels

ABSTRACT

We have studied the effect of the demethylating agent azacytidine (azaC) on expression of a β-glucuronidase (GUS) gene transferred to tobacco leaf disks by Agrobacterium-mediated transformation. In a system where no selection was performed, where shoot formation was partially repressed, and where Agrobacterium does not express the GUS gene, we were able to follow the early events of transient and stable expression. Two days after inoculation, 8% of the cells expressed GUS but this proportion rapidly decreased to near zero in the following week. Treatment of leaf disks with azaC just after transformation retarded this inactivation to some extent, while treatment of Agrobacterium prior to transformation increased the frequency of transient expression. Three weeks after inoculation the number of GUS-expressing cells increased 4- to 6-fold in the leaf disks treated with azaC and in the leaf disks transformed with azaC-treated bacteria, while the control remained low. These data suggest that DNA methylation is involved in transgene inactivation and that a large number of silent but potentially active transgenes become integrated. More... »

PAGES

429-435

References to SciGraph publications

  • 1989-04. Rapid induction of genomic demethylation and T-DNA gene expression in plant cells by 5-azacytosine derivatives in PLANT MOLECULAR BIOLOGY
  • 1990-01. Construction of an intron-containing marker gene: Splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation in MOLECULAR GENETICS AND GENOMICS
  • 1991-10. Reactivation of a silent Ac following tissue culture is associated with heritable alterations in its methylation pattern in MOLECULAR GENETICS AND GENOMICS
  • 1990-12. The effect of T-DNA copy number, position and methylation on reporter gene expression in tobacco transformants in PLANT MOLECULAR BIOLOGY
  • 1987-12. Assaying chimeric genes in plants: The GUS gene fusion system in PLANT MOLECULAR BIOLOGY REPORTER
  • 1988-10. DNA methylation of the maize transposable element Ac interferes with its transcription in MOLECULAR GENETICS AND GENOMICS
  • 1990-02. A method for early detection of T-DNA transfer in PLANT MOLECULAR BIOLOGY REPORTER
  • 1990-01. Localized transient expression of GUS in leaf discs following cocultivation with Agrobacterium in PLANT MOLECULAR BIOLOGY
  • 1984. Gene Activation by 5-Azacytidine in DNA METHYLATION
  • 1984-11. Changes in T-DNA methylation and expression are associated with phenotypic variation and plant regeneration in a crown gall tumor line in MOLECULAR GENETICS AND GENOMICS
  • 1991-04. Effects of 5-azacytidine on transformation and gene expression inNicotiana tabacum in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • 1968-06. 5-Methylcytosine and 6-Methylaminopurine in Bacterial DNA in NATURE
  • 1988-03. DNA methylation and tissue-specific transcription of the storage protein genes of maize in PLANT MOLECULAR BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00028801

    DOI

    http://dx.doi.org/10.1007/bf00028801

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1053085625

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/7680239


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