Isolation of single-copy-sequence clones from a yeast artificial chromosome library of randomly-sheared Arabidopsis thaliana DNA View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-04

AUTHORS

Eric R. Ward, George C. Jen

ABSTRACT

We describe the construction of a yeast artificial chromosome (YAC) library from the Arabidopsis thaliana genome. Randomly sheared high molecular weight source DNA was extracted from frozen, ground leaf tissue and blunt-end-ligated to the vector pYAC3. By size-fractionating the ligation products, we achieved an average clone size of 150 kb. Approximately 6% of the YACs contained inserts from the chloroplast genome. We screened clones equivalent to greater than four A. thaliana haploid nuclear genomes and isolated YACs homologous to five single-copy-sequence probes. The library should be useful chromosome walking and genome mapping experiments. In addition, the approach used for its construction should be applicable to other higher plant species. More... »

PAGES

561-568

References to SciGraph publications

  • 1983-09. Construction of artificial chromosomes in yeast in NATURE
  • 1984-04. The DNA of Arabidopsis thaliana in MOLECULAR GENETICS AND GENOMICS
  • 1988-09. Genome linking with yeast artificial chromosomes in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00027501

    DOI

    http://dx.doi.org/10.1007/bf00027501

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1053373097

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/2102834


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