The use of cultured Acer cells as a screening tool for mitotic inhibitors. I. Reference herbicides View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-02

AUTHORS

Françoise Nurit, Edna Gomez de Melo, Patrick Ravanel, Michel Tissut

ABSTRACT

Several commercially available mitosis inhibitors have been studied for their effects on an Acer pseudoplatanus cell suspension culture; these were colchicine, N-(1,1-dimethylpropynyl)3-chlorobenzamide, propyzamide and trifluralin. The last two compounds are known as preemergence herbicides. In the studied cultures, the concentrations necessary to arrest mitosis were between 50 and 100 μM for colchicine, and between 10 and 100 μM for propyzamide, N-(1,1-dimethylpropynyl)3-chlorobenzamide and trifluralin. However, protein synthesis, plastid and mitochondria division and dry weight increase occurred in the treated cells, leading to the formation of giant cells. These results suggest that mitosis inhibition is the fundamental effect of these compounds at the concentrations studied. At these concentrations, metabolic activities seem unaffected, in contrast with what was found previously in the case of chlorpropham. This phenylcarbamate inhibited mitosis in the same cells at 100 μM, but also prevented protein synthesis and weight increase, probably as a result of its uncoupling properties.The effect of another compound, glyphosate, on Acer cell suspension cultures, was studied concurrently. At concentrations between 10 and 100 μM, it only slightly decreased protein formation in the culture, and was unable to prevent mitosis from occurring.The formation of giant cells in Acer cell suspension cultures seems to be a good criterion to demonstrate that selective mitosis inhibition has taken place. This criterion might be used in the case of mitosis inhibitors acting on the G2-phase. More... »

PAGES

47-57

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00025278

DOI

http://dx.doi.org/10.1007/bf00025278

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1027786033


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