Polyacrylamide gradient-gel electrophoresis: a routine method for high resolution isozyme electrophoresis ofSolanum andLycopersicon species View Full Text


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Article Info

DATE

1989-02

AUTHORS

L. C. J. M. Suurs, E. Jongedijk, M. M. C. Tan

ABSTRACT

This paper describes a refined method for isozyme analysis based upon polyacrylamide gel electrophoresis. The method could be routinely used and clearly reproducible banding patterns were obtained forPgdh, Pgm, Pgi andGot inLycopersicon peruvianum, Solanum lycopersicoides, Solanum pennellii and tuberbearingSolanum species hybrids. When compared to starch gel electrophoresis, the present method is superior in giving more constant and high quality bands with a better resolution of isozyme variants. In addition, pore size and pH gradients may be applied within a single gel to optimize band separation and resolution. This is of particular importance when there are only subtle differences in the migrational capacities of different isozyme variants which cannot be otherwise resolved. More... »

PAGES

181-186

References to SciGraph publications

  • 1970-04. Starch gel electrophoresis of enzymes—A compilation of recipes in BIOCHEMICAL GENETICS
  • 1977-06. Genetic variation inLycopersicon pimpinellifolium: Evidence of evolutionary change in mating systems in PLANT SYSTEMATICS AND EVOLUTION
  • 1984-04. Genetic bases of isozyme variation for alkaline phosphatase and glucosephosphate isomerase in Solanum in THEORETICAL AND APPLIED GENETICS
  • 1985-11. The pattern of megasporogenesis and megagametogenesis in diploid Solanum species hybrids; its relevance to the origin of 2n-eggs and the induction of apomixis in EUPHYTICA
  • 1981-08. Serial electrophoretic transfers: A technique for the identification of numerous enzymes from single polyacrylamide gels in BIOCHEMICAL GENETICS
  • 1969-02. Genetic control of multiple molecular forms of enzymes in plants: A review in BIOCHEMICAL GENETICS
  • 1980-03. Isozymic gene linkage map of the tomato: Applications in genetics and breeding in THEORETICAL AND APPLIED GENETICS
  • 1985-05. Isozyme gene duplication in diploid and tetraploid potatoes in THEORETICAL AND APPLIED GENETICS
  • 1985-05. A genetic classification of potato cultivars based on allozyme patterns in THEORETICAL AND APPLIED GENETICS
  • 1987-08. Relative performance of monohaploid potato clones and their diploid parents at plant level and after protoplast isolation and subsequent fusion in THEORETICAL AND APPLIED GENETICS
  • 1986-02. Somatic hybrid plants between Lycopersicon esculentum and Solanum lycopersicoides in THEORETICAL AND APPLIED GENETICS
  • 1983-06. First division restitution gametes through fertile desynaptic mutants of potato in EUPHYTICA
  • 1985-04. Somatic hybridization between Lycopersicon esculentum and Lycopersicon pennellii in THEORETICAL AND APPLIED GENETICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00024509

    DOI

    http://dx.doi.org/10.1007/bf00024509

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1086071007


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