Developmental, hormonal, and pathogenesis-related regulation of the tobacco class I β-1,3-glucanase B promoter View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1994-05

AUTHORS

Regina Vögeli-Lange, Corinne Fründt, Craig M. Hart, Ferenc Nagy, Frederick Meins

ABSTRACT

The class I β-1,3-glucanases are antifungal vacuolar proteins implicated in plant defense that show developmental, hormonal, and pathogenesis-related regulation. The tobacco enzymes are encoded by a small gene family with members derived from ancestors related to the present-day species Nicotiana sylvestris and N. tomentosiformis. We studied the expression in transgenic tobacco plants of a chimeric β-glucuronidase (GUS) reporter gene fused to 1.6 kb of upstream sequence of the tobacco class I β-1,3-glucanase B (GLB) gene, which is of N. tomentosiformis origin. Expression of the GUS reporter gene and the accumulation of class I β-1,3-glucanase and its mRNA showed very similar patterns of regulation. In young seedlings the reporter gene was expressed in the roots. In mature tobacco plants it was preferentially expressed in lower leaves and roots and was induced in leaves by ethylene treatment and by infection with tobacco mosaic virus (TMV). Furthermore, it was down-regulated in cultured leaf discs by combinations of the hormones auxin and cytokinin. Histological studies of GUS activity showed that the GLB promoter shows highly localized expression in roots of seedlings. It is also expressed in a ring of cells around necrotic lesions induced by TMV infection, but not in cells immediately adjacent to the lesions or in the lesions themselves. The results of deletion analyses suggest that multiple positive and negative elements in the GLB promoter regulate its activity. The region from −1452 to −1193 containing two copies of the heptanucleotide AGCCGCC, which is highly conserved in plant-stress and defense-related genes, is necessary for high level expression in leaves. Additional regions important for organ-specific and regulated expression were: −568 to −402 for ethylene induction of leaves; −402 to −211 for expression in lower leaves and cultured leaf discs and for TMV induction of leaves; and −211 to −60 for expression in roots. More... »

PAGES

299-311

References to SciGraph publications

  • 1985-02. Tobacco single-copy DNA is highly homologous to sequences present in the genomes of its diploid progenitors in MOLECULAR GENETICS AND GENOMICS
  • 1993-01. A 61 bp enhancer element of the tobacco β-1,3-glucanase B gene interacts with one or more regulated nuclear proteins in PLANT MOLECULAR BIOLOGY
  • 1990-12. Structure and expression of a tobacco β-1,3-glucanase gene in PLANT MOLECULAR BIOLOGY
  • 1990-12. The effect of T-DNA copy number, position and methylation on reporter gene expression in tobacco transformants in PLANT MOLECULAR BIOLOGY
  • 1985-06. Purification, immunoassay and characterization of an abundant, cytokinin-regulated polypeptide in cultured tobacco tissues in PLANTA
  • 1990-08. The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leaves in PLANTA
  • 1992-04. The structure and regulation of homeologous tobacco endochitinase genes of Nicotiana sylvestris and N. tomentosiformis origin in MOLECULAR GENETICS AND GENOMICS
  • 1987-11. Ethylene regulation of β-1,3-glucanase in tobacco in PLANTA
  • 1992. The Primary Structure of Plant Pathogenesis-related Glucanohydrolases and Their Genes in GENES INVOLVED IN PLANT DEFENSE
  • 1969-12. Cell-wall-hydrolysing enzymes in wall formation as measured by pollen-tube extension in PLANTA
  • 1992-11. Ozone-induced changes of mRNA levels of β-1,3-glucanase, chitinase and ‘pathogenesis-related’ protein 1b in tobacco plants in PLANT MOLECULAR BIOLOGY
  • 1993-02. Analysis of regulatory elements involved in stress-induced and organ-specific expression of tobacco acidic and basic β-1,3-glucanase genes in PLANT MOLECULAR BIOLOGY
  • 1990-02. Tobacco genes encoding acidic and basic isoforms of pathogenesis-related proteins display different expression patterns in PLANT MOLECULAR BIOLOGY
  • 1986-02. Developmental and hormonal regulation of β-1,3-glucanase in tobacco in PLANTA
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00023245

    DOI

    http://dx.doi.org/10.1007/bf00023245

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1010790773

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/8018877


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