Hybrid Tea-rose pollen. I. Germination and storage View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1977-12

AUTHORS

T. Visser, D. P. De Vries, G. W. H. Welles, J. A. M. Scheurink

ABSTRACT

Germination and storage trials were carried out with pollen of several rose varieties. The pollen grains germinated well in a 15% sucrose solution with 40 ppm boric acid. Staining the pollen with a 0.1% tetrazolium solution and standardizing the degree of colour at which the pollen grains are counted as viable, provided a good viability estimate, simpler to carry out than in vitro germination. Germination capacity and staining ability of the pollen were greatly impeded-about halved-by dehydration during storage in desiccators at low humidity. This effect could be corrected by humidifying the pollen beforehand for about one hour, though this pre-treatment increased the percentage of germinated pollen grains more than the percentage stained. There was no difference between the two percentages in fresh or in deep-frozen pollen. Pollen stored at 1°C and high relative humidity soon lost its germination capacity: between 0 and 20% humidity a considerable proportion of the pollen remained viable for 9 months and longer. Storage for the same period in vacuum-sealed glass tubes at −24°C maintained viability as well or better and would probably prolong it further. Some of the cold-stored pollen induced a reasonable seed set after one year, a low seed set was obtained even after two years of storage at 1°C and low humidity. More... »

PAGES

721-728

References to SciGraph publications

Journal

TITLE

Euphytica

ISSUE

3

VOLUME

26

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00021697

DOI

http://dx.doi.org/10.1007/bf00021697

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1031580689


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