Cloning, sequence analysis and expression of a cDNA encoding active phosphoenolpyruvate carboxylase of the C3 plant Solanum tuberosum View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-11

AUTHORS

Sabine Merkelbach, Johanna Gehlen, Martin Denecke, Heinz-Josef Hirsch, Fritz Kreuzaler

ABSTRACT

A cDNA coding for phosphoenolpyruvate carboxylase (PEPC) was isolated from a cDNA library from Solanum tuberosum and the sequence of the cDNA was determined. It was inserted into a bacterial expression vector and a PEPC- Escherichia coli mutant could be complemented by the cDNA construct. A functional fusion protein could be synthesized in E. coli. The properties of this PEPC protein clearly resembled those of typical C3 plant enzymes. More... »

PAGES

881-888

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00021542

DOI

http://dx.doi.org/10.1007/bf00021542

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029153895

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8251640


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