A chimaeric hygromycin resistance gene as a selectable marker in plant cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1985-09

AUTHORS

Peter J. M. van den Elzen, Jeffrey Townsend, Kathleen Y. Lee, John R. Bedbrook

ABSTRACT

We show here that plant cells are sensitive to the antibiotic hygromycin-B(4). We also show that a chimaeric gene consisting of the nopaline synthase (nos) gene regulatory elements and the E. coli derived hygromycin phosphotransferase (hpt) gene, when transferred to plants' cells, confers resistance to hygromycin B. The chimaeric nos-hpt gene enables efficient selection of DNA transfer to plant cells when used in conjunction with Ti plasmid-derived binary vectors in cocultivation experiments. More... »

PAGES

299-302

Journal

TITLE

Plant Molecular Biology

ISSUE

5

VOLUME

5

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00020627

    DOI

    http://dx.doi.org/10.1007/bf00020627

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1049425617

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24306921


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