Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1985-03

AUTHORS

Scott O. Rogers, Arnold J. Bendich

ABSTRACT

We have developed a DNA extraction procedure for milligram amounts of plant tissue. Yields ranged from 0.3-200 nanograms of DNA per milligram of tissue. The factors affecting yield are discussed. Fresh tissue, as well as herbarium specimens (22-118 years old) and mummified seeds and embryos (500 to greater than 44 600 years old) were used. All tissues attempted (57 types from 29 species) yielded measurable amounts of DNA. In no case tested was inhibition observed for restriction enzymes BamHI or EcoRI. More... »

PAGES

69-76

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00020088

DOI

http://dx.doi.org/10.1007/bf00020088

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1047432473

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24306565


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