Variations during leaf development of the relative amounts of two bean (Phaseolus vulgaris) chloroplast tRNAsPhe which differ in their minor ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-06

AUTHORS

H. Pfitzinger, L. Maréchal-Drouard, D. T. N. Pillay, J. H. Weil, P. Guillemaut

ABSTRACT

Bean (Phaseolus vulgaris cv. Saxa) chloroplasts contain two tRNA(Phe) species, namely tRNA(Phe)1 and tRNA(Phe)2. By sequence determination, we show that tRNA(Phe)2 is identical to the previously sequenced tRNA(Phe)1 except for two undermodified nucleotides. By reversed-phase chromatography analyses, we demonstrate that the relative amounts of these two chloroplast tRNAs(Phe) vary during leaf development: in etiolated leaves the undermodified tRNA(Phe)2 only represents 15% of total chloroplast tRNA(Phe), during development and greening it increases to reach 60% in 8-day-old leaves, and it then decreases to 9% in senescing leaves. More... »

PAGES

969-975

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00019393

DOI

http://dx.doi.org/10.1007/bf00019393

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1052704692

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/2102879


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