Activity of a maize ubiquitin promoter in transgenic rice View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-11

AUTHORS

Maria-Jesús Cornejo, Diane Luth, Kathleen M. Blankenship, Olin D. Anderson, Ann E. Blechl

ABSTRACT

We have used the maize ubiquitin 1 promoter, first exon and first intron (UBI) for rice (Oryza sativa L. cv. Taipei 309) transformation experiments and studied its expression in transgenic calli and plants. UBI directed significantly higher levels of transient gene expression than other promoter/intron combinations used for rice transformation. We exploited these high levels of expression to identify stable transformants obtained from callus-derived protoplasts co-transfected with two chimeric genes. The genes consisted of UBI fused to the coding regions of the uidA and bar marker genes (UBI:GUS and UBI:BAR). UBI:GUS expression increased in response to thermal stress in both transfected protoplasts and transgenic rice calli. Histochemical localization of GUS activity revealed that UBI was most active in rapidly dividing cells. This promoter is expressed in many, but not all, rice tissues and undergoes important changes in activity during the development of transgenic rice plants. More... »

PAGES

567-581

References to SciGraph publications

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00019304

    DOI

    http://dx.doi.org/10.1007/bf00019304

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1023000091

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/8219091


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