Isolation and sequence analysis of a nuclear tRNAmet-i gene from soybean View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1987-01

AUTHORS

Julie M. Palmer, William R. Folk

ABSTRACT

A gene encoding the initiator methionine transfer RNA (tRNA(met-i)) has been isolated from a soybean genomic library. The nucleotide sequence of the gene is colinear with the previously determined sequence of bean and wheat germ tRNA(met-i) (3, 8) except for a transversion at site 51/63 in stem IV. Microinjection of the soybean gene into Xenopus oocytes caused the transcription of an RNA product equal in size to mature Xenopus tRNA(met-i). Hybridization of a restriction fragment containing the tRNA(met-i) gene to digests of soybean DNA reveals that the gene occurs in only one to a few copies per haploid genome. More... »

PAGES

47-51

References to SciGraph publications

  • 1980. Surrogate Genetics in the Frog Oocyte in TRANSFER OF CELL CONSTITUENTS INTO EUKARYOTIC CELLS
  • 1978-02. DNA sequence organization in the soybean plant in BIOCHEMICAL GENETICS
  • 1981-03. Differential usage of iso-accepting tRNASer species in silk glands of Bombyx mori in NATURE
  • Journal

    TITLE

    Plant Molecular Biology

    ISSUE

    1

    VOLUME

    8

    Author Affiliations

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00016433

    DOI

    http://dx.doi.org/10.1007/bf00016433

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1028077733

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24302523


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