Agroinfection and nucleotide sequence of cloned wheat dwarf virus DNA View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1988-01

AUTHORS

Crispin J. Woolston, Richard Barker, Helen Gunn, Margaret I. Boulton, Philip M. Mullineaux

ABSTRACT

Cloned DNA of the geminivirus wheat dwarf virus (WDV) was successfully used to infect seedling wheat plants. The clone was derived from circular double-stranded viral DNA isolated from naturally infected tissue. The initiation of infection was mediated by Agrobacterium tumefaciens using cloned dimeric WDV genomes in a binary Agrobacterium vector. The WDV DNA which comprised the infectious clone was sequenced and is compared with the published sequence of a Swedish isolate of the same virus. The results confirm that the single WDV genome component of 2.75 kb carries all the information necessary for production of viral symptoms, virus particles and viral double- and single-stranded DNA forms. More... »

PAGES

35-43

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00016012

DOI

http://dx.doi.org/10.1007/bf00016012

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1008055205

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24272156


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