Physical mapping of a region in the soybean (Glycine max) genome containing duplicated sequences View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-06

AUTHORS

Roel P. Funke, Alexander Kolchinsky, Peter M. Gresshoff

ABSTRACT

Pulsed-field gel electrophoresis (PFGE) was used to study a cluster of molecular markers in the soybean genome. There were 550 kb per centimorgan (cM) in the cluster, which is close to the calculated average for the whole genome. The analysis was complicated by the presence of duplicated sequences, and some ambiguities arising from this were resolved by using second-dimension conventional electrophoresis to relate physical maps to the RFLP map of soybean. The results show that there is a high degree of conservation of 'rare cutter' sites between homoeologous regions. Finally, PFGE can confirm physical linkage of monomorphic copies of markers, which can aid in the study and comparison of homoeologous regions that are invisible to RFLP analysis. More... »

PAGES

437-446

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00015974

DOI

http://dx.doi.org/10.1007/bf00015974

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1017458438

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8329683


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