Ontology type: schema:ScholarlyArticle
1991-05
AUTHORS ABSTRACTIn a previous study on doubly transformed tobacco plants, we observed the unexpected inactivation in trans of T-DNA-I (encoding KanrNOS) following the introduction into the same genome of an unlinked copy of T-DNA-II (encoding HygrOCS). This inactivation, which probably resulted from interactions between homologous regions on each T-DNA, was correlated with methylation in the nospro, which controlled the expression of both the nptII and nos genes. In this paper, we show that the inactivation and methylation of the nospronptII gene in the presence of a suppressor T-DNA-II locus can be either complete (epistasis) or partial (cellular mosaicism). In plants showing partial suppression, the strength of the Kanr phenotype, which apparently reflected the proportion of cells expressing the nptII gene, was inversely correlated with the degree of methylation of the nospro. The extent of nospro methylation decreased progressively in successive generations as suppressor T-DNA-II loci were crossed out. The strength of the Kanr phenotype was improved and nospro methylation was less extensive in first generation Kanr progeny obtained from outcrossing with untransformed tobacco than from self-fertilization. More... »
PAGES821-830
http://scigraph.springernature.com/pub.10.1007/bf00015074
DOIhttp://dx.doi.org/10.1007/bf00015074
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1039962530
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/1650257
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