The small, versatilepPZP family ofAgrobacterium binary vectors for plant transformation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1994-09

AUTHORS

Peter Hajdukiewicz, Zora Svab, Pal Maliga

ABSTRACT

The new pPZP Agrobacterium binary vectors are versatile, relatively small, stable and are fully sequenced. The vectors utilize the pTiT37 T-DNA border regions, the pBR322 bom site for mobilization from Escherichia coli to Agrobacterium, and the ColE1 and pVS1 plasmid origins for replication in E. coli and in Agrobacterium, respectively. Bacterial marker genes in the vectors confer resistance to chloramphenicol (pPZP100 series) or spectinomycin (pPZP200 series), allowing their use in Agrobacterium strains with different drug resistance markers. Plant marker genes in the binary vectors confer resistance to kanamycin or to gentamycin, and are adjacent to the left border (LB) of the transferred region. A lacZ alpha-peptide, with the pUC18 multiple cloning site (MCS), lies between the plant marker gene and the right border (RB). Since the RB is transferred first, drug resistance is obtained only if the passenger gene is present in the transgenic plants. More... »

PAGES

989-994

Journal

TITLE

Plant Molecular Biology

ISSUE

6

VOLUME

25

Author Affiliations

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    URI

    http://scigraph.springernature.com/pub.10.1007/bf00014672

    DOI

    http://dx.doi.org/10.1007/bf00014672

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1007506198

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/7919218


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