Structure and function of selectable and non-selectable transgenes in maize after introduction by particle bombardment View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1994-09

AUTHORS

James C. Register, David J. Peterson, Philip J. Bell, W. Paul Bullock, Ian J. Evans, Bronwyn Frame, Andrew J. Greenland, Nicole S. Higgs, Ian Jepson, Shuping Jiao, Carol J. Lewnau, Jeff M. Sillick, H. Martin Wilson

ABSTRACT

Zea mays transformants produced by particle bombardment of embryogenic suspension culture cells of the genotype A188 × B73 and selected on kanamycin or bialaphos were characterized with respect to transgene integration, expression, and inheritance. Selection on bialaphos, mediated by thebar orpat genes, was more efficient than selection on kanamycin, mediated by thenptII gene. Most transformants contained multicopy, single locus, transgene insertion events. A transgene expression cassette was more likely to be rearranged if expression of that gene was not selected for during callus growth. Not all plants regenerated from calli representing single transformation events expressed the transgenes, and a non-selectable gene (uidA) was expressed in fewer plants than was the selectable transgene. Mendelian inheritance of transgenes consistent with transgene insertion at a single locus was observed for approximately two thirds of the transformants assessed. Transgene expression was typically, but not always, predictable in progeny plants-transgene silencing, as well as poor transgene transmission to progeny, was observed in some plant lines in which the parent plants had expressed the transgene. More... »

PAGES

951-961

References to SciGraph publications

  • 1990-09-01. Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants in BIO/TECHNOLOGY
  • 1987-12. Assaying chimeric genes in plants: The GUS gene fusion system in PLANT MOLECULAR BIOLOGY REPORTER
  • 1988. Extraction of DNA from plant tissues in PLANT MOLECULAR BIOLOGY MANUAL
  • 1990-02. Transgenic tobacco plants and their progeny derived by microprojectile bombardment of tobacco leaves in PLANT MOLECULAR BIOLOGY
  • 1993-02-01. Field Performance of Elite Transgenic Maize Plants Expressing an Insecticidal Protein Derived from Bacillus thuringiensis in NATURE BIOTECHNOLOGY
  • 1989-11. Characterization of a pollen-specific genomic clone from maize in PLANT REPRODUCTION
  • 1987-09. A simple and rapid method for minipreparation of DNA from tissue cultured plant cells in PLANT MOLECULAR BIOLOGY REPORTER
  • 1993-02. Activity of a chimeric promoter with the doubled CaMV 35S enhancer element in protoplast-derived cells and transgenic plants in maize in PLANT MOLECULAR BIOLOGY
  • 1985-05. Molecular and general genetics of a hybrid foreign gene introduced into tobacco by direct gene transfer in MOLECULAR GENETICS AND GENOMICS
  • 1988-05. Expression and inheritance of kanamycin resistance in a large number of transgenic petunias generated by Agrobacterium-mediated transformation in PLANT MOLECULAR BIOLOGY
  • 1992-01. Dissection of a pollen-specific promoter from maize by transient transformation assays in PLANT MOLECULAR BIOLOGY
  • 1985-05. Establishment and maintenance of friable, embryogenic maize callus and the involvement of L-proline in PLANTA
  • 1987-06. Covalent DNA modification and the regulation of Mutator element transposition in maize in MOLECULAR GENETICS AND GENOMICS
  • 1992-01. Transformation and inheritance of a hygromycin phosphotransferase gene in maize plants in PLANT MOLECULAR BIOLOGY
  • 1992-01. Segregation of transgenes in maize in PLANT MOLECULAR BIOLOGY
  • 1988-05. Analysis of the T-DNA structure in a large number of transgenic petunias generated by Agrobacterium-mediated transformation in PLANT MOLECULAR BIOLOGY
  • 1988-05-01. Factors Influencing Gene Delivery into Zea Mays Cells by High–Velocity Microprojectiles in NATURE BIOTECHNOLOGY
  • 1989-06-01. Regeneration of Fertile Plants from Protoplasts of Elite Inbread Maize. in NATURE BIOTECHNOLOGY
  • Journal

    TITLE

    Plant Molecular Biology

    ISSUE

    6

    VOLUME

    25

    Related Patents

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  • Genetic Engineering
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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00014669

    DOI

    http://dx.doi.org/10.1007/bf00014669

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1051372329

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/7919215


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