Amplification and Sequence Analysis of TPI Gene, a Structural Gene of Operon from Lactobacillus delbrueckii View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2015

AUTHORS

Amit Kumar , T. Pravin Reddy , Dhatrika Sahithi

ABSTRACT

The main objective of this work is amplification and sequence analysis of TPI gene which is a structural gene of operon from Lactobacillus delbrueckii. The cow milk was collected under good conditions and analyzed for bacterial load. Many strains of lactic acid bacteria were obtained, out of which 3 strains were homofermentative lactobacilli. Different biochemical tests were done for the identification of Lactobacillus delbrueckii. The TPI gene of Lactobacillus delbrueckii is involved in the maximum production of bacteriocin. Genomic DNA was isolated from Lactobacillus delbrueckii and its TPI gene was successfully amplified using polymerase chain reaction. Further, sequence analysis of TPI gene was done using the bioinformatics tools and the phylogenetic study was made. From the sequence analysis we can infer that the TPI gene sequence of Lactobacillus delbrueckii has close resemblance with the Escherichia coli sequence. More... »

PAGES

1-9

References to SciGraph publications

  • 2003-08-01. A Short History of the Polymerase Chain Reaction in PCR PROTOCOLS
  • 2005-10. Bacteriocins: developing innate immunity for food in NATURE REVIEWS MICROBIOLOGY
  • Book

    TITLE

    Biotechnology and Bioforensics

    ISBN

    978-981-287-049-0
    978-981-287-050-6

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/978-981-287-050-6_1

    DOI

    http://dx.doi.org/10.1007/978-981-287-050-6_1

    DIMENSIONS

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