Plant Tissue Culture for In Vitro Mutagenesis, Large-Scale Propagation, and Genetic Transformation View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2016-10-09

AUTHORS

Pratibha Misra , Syed Saema

ABSTRACT

An ever-increasing demand of uniform plants of commercially valuable plant species needs their clonal propagation on a large scale using different strategies of tissue culture. We have reported a large number of plants which were propagated in vitro using different plant parts. A number of problems were solved before large-scale propagation of these crops, like shoot organogenesis, multiplication of shoots, rooting, acclimatization and hardening, control of shoot browning/necrosis and defoliation, etc. Besides, popularization of temperate climate crops, e.g., Asiatic hybrid lilies and gerbera, to subtropical climate of Lucknow, was achieved using tissue culture. New varieties of chrysanthemum differing in color and shape of the florets were developed using in vitro mutagenesis.Transformation protocols using Agrobacterium tumefaciens have been optimized in Jatropha curcas—a plant important for biofuel and Withania somnifera—an important Indian medicinal plant using leaf segments (LS). W. somnifera has some medicinally important sterol glycosyltransferases (SGT) which are the enzymes that glycosylate sterols and play an important role in providing tolerance to the plant against biotic and abiotic stresses. These genes were differentially expressed in different organs of the plant and also in response to biotic and abiotic stress. Functional characterization of WsSGT gene was done by its overexpression in homologous and heterologous expression systems of N. tabacum and A. thaliana, whereas for suppression/gene silencing, RNAi and artificial miRNA technologies were used. The transgenic plants showed improved germination and tolerance to salt, heat, and cold stress when compared to WT plants. Increased enzyme activity and sterol glycosides could be demonstrated through biochemical analysis. Stress-responsive elements were observed when the promoter of WsSGTL1 gene of W. somnifera was cloned and sequenced. More... »

PAGES

309-342

Book

TITLE

Plant Tissue Culture: Propagation, Conservation and Crop Improvement

ISBN

978-981-10-1916-6
978-981-10-1917-3

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-981-10-1917-3_14

DOI

http://dx.doi.org/10.1007/978-981-10-1917-3_14

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1028394789


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/07", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Agricultural and Veterinary Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Plant Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0703", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Crop and Pasture Production", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "CSIR, National Botanical Research Institute, 226001, Lucknow, India", 
          "id": "http://www.grid.ac/institutes/grid.417642.2", 
          "name": [
            "CSIR, National Botanical Research Institute, 226001, Lucknow, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Misra", 
        "givenName": "Pratibha", 
        "id": "sg:person.01260725315.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01260725315.01"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "CSIR, National Botanical Research Institute, 226001, Lucknow, India", 
          "id": "http://www.grid.ac/institutes/grid.417642.2", 
          "name": [
            "CSIR, National Botanical Research Institute, 226001, Lucknow, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Saema", 
        "givenName": "Syed", 
        "id": "sg:person.014165422346.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014165422346.03"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2016-10-09", 
    "datePublishedReg": "2016-10-09", 
    "description": "An ever-increasing demand of uniform plants of commercially valuable plant species needs their clonal propagation on a large scale using different strategies of tissue culture. We have reported a large number of plants which were propagated in vitro using different plant parts. A number of problems were solved before large-scale propagation of these crops, like shoot organogenesis, multiplication of shoots, rooting, acclimatization and hardening, control of shoot browning/necrosis and defoliation, etc. Besides, popularization of temperate climate crops, e.g., Asiatic hybrid lilies and gerbera, to subtropical climate of Lucknow, was achieved using tissue culture. New varieties of chrysanthemum differing in color and shape of the florets were developed using in vitro mutagenesis.Transformation protocols using Agrobacterium tumefaciens have been optimized in Jatropha curcas\u2014a plant important for biofuel and Withania somnifera\u2014an important Indian medicinal plant using leaf segments (LS). W. somnifera has some medicinally important sterol glycosyltransferases (SGT) which are the enzymes that glycosylate sterols and play an important role in providing tolerance to the plant against biotic and abiotic stresses. These genes were differentially expressed in different organs of the plant and also in response to biotic and abiotic stress. Functional characterization of WsSGT gene was done by its overexpression in homologous and heterologous expression systems of N. tabacum and A. thaliana, whereas for suppression/gene silencing, RNAi and artificial miRNA technologies were used. The transgenic plants showed improved germination and tolerance to salt, heat, and cold stress when compared to WT plants. Increased enzyme activity and sterol glycosides could be demonstrated through biochemical analysis. Stress-responsive elements were observed when the promoter of WsSGTL1 gene of W. somnifera was cloned and sequenced.", 
    "editor": [
      {
        "familyName": "Anis", 
        "givenName": "Mohammad", 
        "type": "Person"
      }, 
      {
        "familyName": "Ahmad", 
        "givenName": "Naseem", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-981-10-1917-3_14", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-981-10-1916-6", 
        "978-981-10-1917-3"
      ], 
      "name": "Plant Tissue Culture: Propagation, Conservation and Crop Improvement", 
      "type": "Book"
    }, 
    "keywords": [
      "Sterol glycosyltransferases", 
      "abiotic stresses", 
      "large-scale propagation", 
      "leaf segments", 
      "artificial miRNA technology", 
      "stress-responsive elements", 
      "valuable plant species", 
      "plant tissue culture", 
      "heterologous expression system", 
      "tissue culture", 
      "important Indian medicinal plant", 
      "Asiatic hybrid lilies", 
      "different plant parts", 
      "WsSGTL1 gene", 
      "WT plants", 
      "transgenic plants", 
      "multiplication of shoots", 
      "plant species", 
      "genetic transformation", 
      "miRNA technology", 
      "Agrobacterium tumefaciens", 
      "gene silencing", 
      "uniform plants", 
      "shoot organogenesis", 
      "Vitro Mutagenesis", 
      "functional characterization", 
      "transformation protocol", 
      "clonal propagation", 
      "expression system", 
      "plant parts", 
      "cold stress", 
      "new varieties", 
      "hybrid lilies", 
      "plants", 
      "genes", 
      "Jatropha curcas", 
      "biochemical analysis", 
      "subtropical climate", 
      "mutagenesis", 
      "enzyme activity", 
      "sterol glycosides", 
      "crops", 
      "different organs", 
      "Withania somnifera", 
      "medicinal plants", 
      "Indian medicinal plants", 
      "somnifera", 
      "thaliana", 
      "tolerance", 
      "important role", 
      "tabacum", 
      "tumefaciens", 
      "RNAi", 
      "glycosylate", 
      "silencing", 
      "organogenesis", 
      "glycosyltransferases", 
      "germination", 
      "shoots", 
      "promoter", 
      "gerbera", 
      "species", 
      "defoliation", 
      "overexpression", 
      "chrysanthemum", 
      "florets", 
      "stress", 
      "curcas", 
      "enzyme", 
      "lilies", 
      "rooting", 
      "culture", 
      "acclimatization", 
      "biofuels", 
      "large number", 
      "different strategies", 
      "climate", 
      "large scale", 
      "Lucknow", 
      "organs", 
      "role", 
      "characterization", 
      "activity", 
      "variety", 
      "protocol", 
      "technology", 
      "demand", 
      "number", 
      "glycosides", 
      "response", 
      "multiplication", 
      "segments", 
      "propagation", 
      "control", 
      "strategies", 
      "color", 
      "salt", 
      "analysis", 
      "elements", 
      "necrosis", 
      "transformation", 
      "part", 
      "popularization", 
      "shape", 
      "scale", 
      "system", 
      "number of problems", 
      "heat", 
      "problem", 
      "hardening"
    ], 
    "name": "Plant Tissue Culture for In Vitro Mutagenesis, Large-Scale Propagation, and Genetic Transformation", 
    "pagination": "309-342", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1028394789"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-981-10-1917-3_14"
        ]
      }
    ], 
    "publisher": {
      "name": "Springer Nature", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-981-10-1917-3_14", 
      "https://app.dimensions.ai/details/publication/pub.1028394789"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2022-05-20T07:46", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/chapter/chapter_368.jsonl", 
    "type": "Chapter", 
    "url": "https://doi.org/10.1007/978-981-10-1917-3_14"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-981-10-1917-3_14'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-981-10-1917-3_14'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-981-10-1917-3_14'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-981-10-1917-3_14'


 

This table displays all metadata directly associated to this object as RDF triples.

194 TRIPLES      23 PREDICATES      138 URIs      128 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-981-10-1917-3_14 schema:about anzsrc-for:06
2 anzsrc-for:0604
3 anzsrc-for:0607
4 anzsrc-for:07
5 anzsrc-for:0703
6 schema:author Nf6ebeceff13042afb37d3d4d3dbf4ed9
7 schema:datePublished 2016-10-09
8 schema:datePublishedReg 2016-10-09
9 schema:description An ever-increasing demand of uniform plants of commercially valuable plant species needs their clonal propagation on a large scale using different strategies of tissue culture. We have reported a large number of plants which were propagated in vitro using different plant parts. A number of problems were solved before large-scale propagation of these crops, like shoot organogenesis, multiplication of shoots, rooting, acclimatization and hardening, control of shoot browning/necrosis and defoliation, etc. Besides, popularization of temperate climate crops, e.g., Asiatic hybrid lilies and gerbera, to subtropical climate of Lucknow, was achieved using tissue culture. New varieties of chrysanthemum differing in color and shape of the florets were developed using in vitro mutagenesis.Transformation protocols using Agrobacterium tumefaciens have been optimized in Jatropha curcas—a plant important for biofuel and Withania somnifera—an important Indian medicinal plant using leaf segments (LS). W. somnifera has some medicinally important sterol glycosyltransferases (SGT) which are the enzymes that glycosylate sterols and play an important role in providing tolerance to the plant against biotic and abiotic stresses. These genes were differentially expressed in different organs of the plant and also in response to biotic and abiotic stress. Functional characterization of WsSGT gene was done by its overexpression in homologous and heterologous expression systems of N. tabacum and A. thaliana, whereas for suppression/gene silencing, RNAi and artificial miRNA technologies were used. The transgenic plants showed improved germination and tolerance to salt, heat, and cold stress when compared to WT plants. Increased enzyme activity and sterol glycosides could be demonstrated through biochemical analysis. Stress-responsive elements were observed when the promoter of WsSGTL1 gene of W. somnifera was cloned and sequenced.
10 schema:editor N0862b1e0a393447794d351ab267aaf70
11 schema:genre chapter
12 schema:inLanguage en
13 schema:isAccessibleForFree false
14 schema:isPartOf N6b4169d3c5624876aef1dce340ba7c4b
15 schema:keywords Agrobacterium tumefaciens
16 Asiatic hybrid lilies
17 Indian medicinal plants
18 Jatropha curcas
19 Lucknow
20 RNAi
21 Sterol glycosyltransferases
22 Vitro Mutagenesis
23 WT plants
24 Withania somnifera
25 WsSGTL1 gene
26 abiotic stresses
27 acclimatization
28 activity
29 analysis
30 artificial miRNA technology
31 biochemical analysis
32 biofuels
33 characterization
34 chrysanthemum
35 climate
36 clonal propagation
37 cold stress
38 color
39 control
40 crops
41 culture
42 curcas
43 defoliation
44 demand
45 different organs
46 different plant parts
47 different strategies
48 elements
49 enzyme
50 enzyme activity
51 expression system
52 florets
53 functional characterization
54 gene silencing
55 genes
56 genetic transformation
57 gerbera
58 germination
59 glycosides
60 glycosylate
61 glycosyltransferases
62 hardening
63 heat
64 heterologous expression system
65 hybrid lilies
66 important Indian medicinal plant
67 important role
68 large number
69 large scale
70 large-scale propagation
71 leaf segments
72 lilies
73 medicinal plants
74 miRNA technology
75 multiplication
76 multiplication of shoots
77 mutagenesis
78 necrosis
79 new varieties
80 number
81 number of problems
82 organogenesis
83 organs
84 overexpression
85 part
86 plant parts
87 plant species
88 plant tissue culture
89 plants
90 popularization
91 problem
92 promoter
93 propagation
94 protocol
95 response
96 role
97 rooting
98 salt
99 scale
100 segments
101 shape
102 shoot organogenesis
103 shoots
104 silencing
105 somnifera
106 species
107 sterol glycosides
108 strategies
109 stress
110 stress-responsive elements
111 subtropical climate
112 system
113 tabacum
114 technology
115 thaliana
116 tissue culture
117 tolerance
118 transformation
119 transformation protocol
120 transgenic plants
121 tumefaciens
122 uniform plants
123 valuable plant species
124 variety
125 schema:name Plant Tissue Culture for In Vitro Mutagenesis, Large-Scale Propagation, and Genetic Transformation
126 schema:pagination 309-342
127 schema:productId N4ac095ec3fe64d43b2272b3ec4723641
128 Nf55882fe333148d195c2ddb0cf26fb18
129 schema:publisher N87e62e44ebcf480780bf266369c820f7
130 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028394789
131 https://doi.org/10.1007/978-981-10-1917-3_14
132 schema:sdDatePublished 2022-05-20T07:46
133 schema:sdLicense https://scigraph.springernature.com/explorer/license/
134 schema:sdPublisher N9ca8055650d04be99ce5b862d40cfc83
135 schema:url https://doi.org/10.1007/978-981-10-1917-3_14
136 sgo:license sg:explorer/license/
137 sgo:sdDataset chapters
138 rdf:type schema:Chapter
139 N0862b1e0a393447794d351ab267aaf70 rdf:first Ndd5293e2247148f8a6cdacaaea44840c
140 rdf:rest N9f057a475f65476a8609c21d7a13e203
141 N4ac095ec3fe64d43b2272b3ec4723641 schema:name dimensions_id
142 schema:value pub.1028394789
143 rdf:type schema:PropertyValue
144 N6b4169d3c5624876aef1dce340ba7c4b schema:isbn 978-981-10-1916-6
145 978-981-10-1917-3
146 schema:name Plant Tissue Culture: Propagation, Conservation and Crop Improvement
147 rdf:type schema:Book
148 N7d18d7b155c5473ba086ae5c119832b3 rdf:first sg:person.014165422346.03
149 rdf:rest rdf:nil
150 N87e62e44ebcf480780bf266369c820f7 schema:name Springer Nature
151 rdf:type schema:Organisation
152 N9ca8055650d04be99ce5b862d40cfc83 schema:name Springer Nature - SN SciGraph project
153 rdf:type schema:Organization
154 N9f057a475f65476a8609c21d7a13e203 rdf:first Nfd532fb447144cebbf94086e506a853c
155 rdf:rest rdf:nil
156 Ndd5293e2247148f8a6cdacaaea44840c schema:familyName Anis
157 schema:givenName Mohammad
158 rdf:type schema:Person
159 Nf55882fe333148d195c2ddb0cf26fb18 schema:name doi
160 schema:value 10.1007/978-981-10-1917-3_14
161 rdf:type schema:PropertyValue
162 Nf6ebeceff13042afb37d3d4d3dbf4ed9 rdf:first sg:person.01260725315.01
163 rdf:rest N7d18d7b155c5473ba086ae5c119832b3
164 Nfd532fb447144cebbf94086e506a853c schema:familyName Ahmad
165 schema:givenName Naseem
166 rdf:type schema:Person
167 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
168 schema:name Biological Sciences
169 rdf:type schema:DefinedTerm
170 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
171 schema:name Genetics
172 rdf:type schema:DefinedTerm
173 anzsrc-for:0607 schema:inDefinedTermSet anzsrc-for:
174 schema:name Plant Biology
175 rdf:type schema:DefinedTerm
176 anzsrc-for:07 schema:inDefinedTermSet anzsrc-for:
177 schema:name Agricultural and Veterinary Sciences
178 rdf:type schema:DefinedTerm
179 anzsrc-for:0703 schema:inDefinedTermSet anzsrc-for:
180 schema:name Crop and Pasture Production
181 rdf:type schema:DefinedTerm
182 sg:person.01260725315.01 schema:affiliation grid-institutes:grid.417642.2
183 schema:familyName Misra
184 schema:givenName Pratibha
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01260725315.01
186 rdf:type schema:Person
187 sg:person.014165422346.03 schema:affiliation grid-institutes:grid.417642.2
188 schema:familyName Saema
189 schema:givenName Syed
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014165422346.03
191 rdf:type schema:Person
192 grid-institutes:grid.417642.2 schema:alternateName CSIR, National Botanical Research Institute, 226001, Lucknow, India
193 schema:name CSIR, National Botanical Research Institute, 226001, Lucknow, India
194 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...