Cultured-Animal-Cell Assay for Collagen-Synthesis-Activation Factor Produced by Callus of Woody Plant Eucommia Ulmoides View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1998

AUTHORS

Akinori Ono , Xin-Hui Xing , Hajime Unno

ABSTRACT

In order to quantify the undefined factor enhancing collagen synthesis of animal cells (FECS) which is contained in a medicinal woody plant, Eucommia ulmoides and its cultured callus, an assay system using human fibroblast cell (HS-K) capable of synthesizing collagen was established. The HS-K cells were cultured in DMEM medium containing 10% FBS. Extracts of dried leaves and cultured calli of E.ulmoides, and broth of the callus culture were added into the HS-K culture. After 120-h incubation, collagen amount accumulated in the HS-K cells and in the medium was determined by measuring the amount of hydroxyproline obtained from the hydrolysis of the collagen. Intensity of the FECS was evaluated by the relative ratio of the synthesized collagen amount per HS-K cell under the presence of the additives to that of the control. The amount of collagen synthesized by the HS-K cells was found to depend on the amount of the added extracts. The relative intensity of FECS in the leaf extract, callus culture broth and callus extract were 2.5, 2.3 and 1.7, respectively. More... »

PAGES

385-389

Book

TITLE

Animal Cell Technology: Basic & Applied Aspects

ISBN

978-94-010-6170-4
978-94-011-5161-0

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-94-011-5161-0_66

DOI

http://dx.doi.org/10.1007/978-94-011-5161-0_66

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029835200


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Tokyo Institute of Technology", 
          "id": "https://www.grid.ac/institutes/grid.32197.3e", 
          "name": [
            "Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ono", 
        "givenName": "Akinori", 
        "id": "sg:person.012455145640.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012455145640.51"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tokyo Institute of Technology", 
          "id": "https://www.grid.ac/institutes/grid.32197.3e", 
          "name": [
            "Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xing", 
        "givenName": "Xin-Hui", 
        "id": "sg:person.01124373510.98", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01124373510.98"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tokyo Institute of Technology", 
          "id": "https://www.grid.ac/institutes/grid.32197.3e", 
          "name": [
            "Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Unno", 
        "givenName": "Hajime", 
        "id": "sg:person.0730705634.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0730705634.57"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.2302/kjm.27.43", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013841684"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1271/bbb.57.205", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041668333"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1998", 
    "datePublishedReg": "1998-01-01", 
    "description": "In order to quantify the undefined factor enhancing collagen synthesis of animal cells (FECS) which is contained in a medicinal woody plant, Eucommia ulmoides and its cultured callus, an assay system using human fibroblast cell (HS-K) capable of synthesizing collagen was established. The HS-K cells were cultured in DMEM medium containing 10% FBS. Extracts of dried leaves and cultured calli of E.ulmoides, and broth of the callus culture were added into the HS-K culture. After 120-h incubation, collagen amount accumulated in the HS-K cells and in the medium was determined by measuring the amount of hydroxyproline obtained from the hydrolysis of the collagen. Intensity of the FECS was evaluated by the relative ratio of the synthesized collagen amount per HS-K cell under the presence of the additives to that of the control. The amount of collagen synthesized by the HS-K cells was found to depend on the amount of the added extracts. The relative intensity of FECS in the leaf extract, callus culture broth and callus extract were 2.5, 2.3 and 1.7, respectively.", 
    "editor": [
      {
        "familyName": "Nagai", 
        "givenName": "K.", 
        "type": "Person"
      }, 
      {
        "familyName": "Wachi", 
        "givenName": "M.", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-94-011-5161-0_66", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-94-010-6170-4", 
        "978-94-011-5161-0"
      ], 
      "name": "Animal Cell Technology: Basic & Applied Aspects", 
      "type": "Book"
    }, 
    "name": "Cultured-Animal-Cell Assay for Collagen-Synthesis-Activation Factor Produced by Callus of Woody Plant Eucommia Ulmoides", 
    "pagination": "385-389", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1029835200"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-94-011-5161-0_66"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "8e21a9523dfd5f76a7f178786860126bf14daac3d4ee63b88b01d39bd8939ec8"
        ]
      }
    ], 
    "publisher": {
      "location": "Dordrecht", 
      "name": "Springer Netherlands", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-94-011-5161-0_66", 
      "https://app.dimensions.ai/details/publication/pub.1029835200"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2019-04-16T09:22", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130831_00000002.jsonl", 
    "type": "Chapter", 
    "url": "https://link.springer.com/10.1007%2F978-94-011-5161-0_66"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-94-011-5161-0_66'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-94-011-5161-0_66'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-94-011-5161-0_66'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-94-011-5161-0_66'


 

This table displays all metadata directly associated to this object as RDF triples.

90 TRIPLES      23 PREDICATES      29 URIs      20 LITERALS      8 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-94-011-5161-0_66 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N459860d65388486fb5d8f990e9231eb2
4 schema:citation https://doi.org/10.1271/bbb.57.205
5 https://doi.org/10.2302/kjm.27.43
6 schema:datePublished 1998
7 schema:datePublishedReg 1998-01-01
8 schema:description In order to quantify the undefined factor enhancing collagen synthesis of animal cells (FECS) which is contained in a medicinal woody plant, Eucommia ulmoides and its cultured callus, an assay system using human fibroblast cell (HS-K) capable of synthesizing collagen was established. The HS-K cells were cultured in DMEM medium containing 10% FBS. Extracts of dried leaves and cultured calli of E.ulmoides, and broth of the callus culture were added into the HS-K culture. After 120-h incubation, collagen amount accumulated in the HS-K cells and in the medium was determined by measuring the amount of hydroxyproline obtained from the hydrolysis of the collagen. Intensity of the FECS was evaluated by the relative ratio of the synthesized collagen amount per HS-K cell under the presence of the additives to that of the control. The amount of collagen synthesized by the HS-K cells was found to depend on the amount of the added extracts. The relative intensity of FECS in the leaf extract, callus culture broth and callus extract were 2.5, 2.3 and 1.7, respectively.
9 schema:editor N516f7ef70c0046d28af9e5eefd04c19c
10 schema:genre chapter
11 schema:inLanguage en
12 schema:isAccessibleForFree false
13 schema:isPartOf N3d55e59b32bd4a7f846a0c5e3ed92449
14 schema:name Cultured-Animal-Cell Assay for Collagen-Synthesis-Activation Factor Produced by Callus of Woody Plant Eucommia Ulmoides
15 schema:pagination 385-389
16 schema:productId N168c0ec9694a43659defdff4cf349dd3
17 N8195ae174fed4f7887004b0f1dc164c7
18 Nb1adbc8a6add43b5b19a815ce64ff791
19 schema:publisher Nb44d422c720249c38661533ec03a8d7d
20 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029835200
21 https://doi.org/10.1007/978-94-011-5161-0_66
22 schema:sdDatePublished 2019-04-16T09:22
23 schema:sdLicense https://scigraph.springernature.com/explorer/license/
24 schema:sdPublisher N382efe97ae3242849011409e00189c48
25 schema:url https://link.springer.com/10.1007%2F978-94-011-5161-0_66
26 sgo:license sg:explorer/license/
27 sgo:sdDataset chapters
28 rdf:type schema:Chapter
29 N1226ef45f7844ea295d0a4ce34dc3873 rdf:first sg:person.0730705634.57
30 rdf:rest rdf:nil
31 N168c0ec9694a43659defdff4cf349dd3 schema:name readcube_id
32 schema:value 8e21a9523dfd5f76a7f178786860126bf14daac3d4ee63b88b01d39bd8939ec8
33 rdf:type schema:PropertyValue
34 N2d9099eb0c854a6e9b5a43f89947662f rdf:first Ncab4e44438db433cbe3761e744ea646d
35 rdf:rest rdf:nil
36 N382efe97ae3242849011409e00189c48 schema:name Springer Nature - SN SciGraph project
37 rdf:type schema:Organization
38 N3d55e59b32bd4a7f846a0c5e3ed92449 schema:isbn 978-94-010-6170-4
39 978-94-011-5161-0
40 schema:name Animal Cell Technology: Basic & Applied Aspects
41 rdf:type schema:Book
42 N459860d65388486fb5d8f990e9231eb2 rdf:first sg:person.012455145640.51
43 rdf:rest N5820eeb42c2d4dc2833a4fa67d5535f8
44 N516f7ef70c0046d28af9e5eefd04c19c rdf:first N72d95f7540c64b338816d1865a9343fa
45 rdf:rest N2d9099eb0c854a6e9b5a43f89947662f
46 N5820eeb42c2d4dc2833a4fa67d5535f8 rdf:first sg:person.01124373510.98
47 rdf:rest N1226ef45f7844ea295d0a4ce34dc3873
48 N72d95f7540c64b338816d1865a9343fa schema:familyName Nagai
49 schema:givenName K.
50 rdf:type schema:Person
51 N8195ae174fed4f7887004b0f1dc164c7 schema:name dimensions_id
52 schema:value pub.1029835200
53 rdf:type schema:PropertyValue
54 Nb1adbc8a6add43b5b19a815ce64ff791 schema:name doi
55 schema:value 10.1007/978-94-011-5161-0_66
56 rdf:type schema:PropertyValue
57 Nb44d422c720249c38661533ec03a8d7d schema:location Dordrecht
58 schema:name Springer Netherlands
59 rdf:type schema:Organisation
60 Ncab4e44438db433cbe3761e744ea646d schema:familyName Wachi
61 schema:givenName M.
62 rdf:type schema:Person
63 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
64 schema:name Biological Sciences
65 rdf:type schema:DefinedTerm
66 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
67 schema:name Biochemistry and Cell Biology
68 rdf:type schema:DefinedTerm
69 sg:person.01124373510.98 schema:affiliation https://www.grid.ac/institutes/grid.32197.3e
70 schema:familyName Xing
71 schema:givenName Xin-Hui
72 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01124373510.98
73 rdf:type schema:Person
74 sg:person.012455145640.51 schema:affiliation https://www.grid.ac/institutes/grid.32197.3e
75 schema:familyName Ono
76 schema:givenName Akinori
77 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012455145640.51
78 rdf:type schema:Person
79 sg:person.0730705634.57 schema:affiliation https://www.grid.ac/institutes/grid.32197.3e
80 schema:familyName Unno
81 schema:givenName Hajime
82 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0730705634.57
83 rdf:type schema:Person
84 https://doi.org/10.1271/bbb.57.205 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041668333
85 rdf:type schema:CreativeWork
86 https://doi.org/10.2302/kjm.27.43 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013841684
87 rdf:type schema:CreativeWork
88 https://www.grid.ac/institutes/grid.32197.3e schema:alternateName Tokyo Institute of Technology
89 schema:name Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226, Japan
90 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...