A New High-Sensitivity 10-ns Time-Resolution Spectrophotometric Technique Adapted to In Vivo Analysis of the Photosynthetic Apparatus View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1998

AUTHORS

Pierre Joliot , Daniel Béal , Fabrice Rappaport

ABSTRACT

In the past years, we have developed a new type of spectrophotometer in which the absorption is sampled by short monochromatic flashes produced by a xenon flash tube (1–2). These instruments are double-beam spectrophotometers in which the ratio between the measuring and the reference signal is computed for each flash in order to correct for the energy fluctuations of the flashes (3%). The time resolution is determined by the duration of the flashes (2 μs) and the light-induced spectral changes can be analyzed in a time window ranging from 2 μs to 10 s with a signal to noise ratio of about 105. Owing to the high sensitivity of these techniques and to the optical design which allows collection of a large fraction of the light scattered by the biological sample, these spectrophotometers are particularly well suited to the analysis of light induced spectral changes in isolated chloroplasts as well as in unicellular algae. More... »

PAGES

4247-4252

Book

TITLE

Photosynthesis: Mechanisms and Effects

ISBN

978-0-7923-5547-2
978-94-011-3953-3

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-94-011-3953-3_983

DOI

http://dx.doi.org/10.1007/978-94-011-3953-3_983

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1090941149


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