Production of Human Antithrombin-III in a Serum-Free Culture of CHO Cells View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1992

AUTHORS

E. Asakura , T. Yamauchi , Y. Amatsuji , S. Uno , R. Furuta , M. Tsujikawa , T. Tanabe , K. Yokoyama

ABSTRACT

This report shows a method to establish a recombinant CHO cell line that grows and secretes human antithrombin-III (AT-III) in a serum-free medium. At first, serum-dependent cell lines were obtained by introducing AT-III cDNA and following MTX-treatments. Then the cells were transferred to a serum-free medium, GCM001, and cultivated for several weeks. Serum- independent cells were obtained through cultivation in GCM001, and some cell lines showed a little greater productivity of AT-III in GCM001 than serum-dependent, parental cells in serum-containing medium. AT-III produced by both serum-independent and serum-dependent cells had the same specific activity, but heterogeneity in glycosylations was observed between them. More... »

PAGES

345-351

Book

TITLE

Animal Cell Technology: Basic & Applied Aspects

ISBN

978-94-010-5263-4
978-94-011-2844-5

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-94-011-2844-5_47

DOI

http://dx.doi.org/10.1007/978-94-011-2844-5_47

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1032473410


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