Characterization of Cell Growth and Improvement of Attachment Kinetics on Macroporous Microcarriers View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1992

AUTHORS

Jung-Hoe Kim , Hun-Soo Lim , Bong-Kwan Han , Madhusudan V. Peshwa , Wei-Shou Hu

ABSTRACT

Macroporous gelatin microcarriers prepared by calcium carbonate inclusion method were used to cultivate vero and HepG2 cells. Confocal microscopy was used to “optically section” microcarriers and visualize the distribution of cells within them. In most microcarriers, cells were found to populate majority of the region close to the surface and some regions in the interior. However, a significant portion of the interior was not occupied. HepG2 cells, in contrast to vero cells, do not attach to these microcarriers at a satisfactory rate. Modification of the beads by increasing the charge density improved their attachment and subsequent growth. Poor attachment of some cell types has been impeding their growth on these microcarriers. An increase in charge density to improve cell attachment may facilitate the cultivation of these cells on macroporous gelatin microcarriers. More... »

PAGES

77-80

Book

TITLE

Animal Cell Technology: Basic & Applied Aspects

ISBN

978-0-7923-1882-8
978-94-011-2844-5

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-94-011-2844-5_11

DOI

http://dx.doi.org/10.1007/978-94-011-2844-5_11

DIMENSIONS

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