Reverse Genetics as an Approach to Isolate a French Bean Resistance Gene Against Anthracnose View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1993

AUTHORS

A. F. Adam , F. Creusot , J. Grisvard , M. Sevignac , N. Choisne , M. Dron

ABSTRACT

Search for molecular markers (RFLP and RAPD markers) linked to three resistance genes (ARE, RVI and MexII) of Phaseolus vulgaris against Colletotrichum lindemuthianum is being carried out with the aim of isolating one of them by the means of reverse genetic methodology. Four couples of Near Isogenic Lines (NILs) are used to select markers linked to the ARE gene. A genetic map is in progress using a cross involving ARE and RVI resistance genes. Another couple of lines is studied to find markers linked to the MexII resistance gene. Isolation of a resistance gene will be carried out by “megabase” technology. High-molecular-weight (HMW) DNA over 2 Mb in size was isolated. Usefulness of this DNA preparation for physical mapping is illustrated by the construction of a large-scale restriction map encompassing 450 Kb around the PAL2 locus. Work intended for the cloning of large DNA fragments into YAC vectors is in progress. The interaction between Phaseolus vulgaris and the fungus Colletotrichum lindemuthianum (the causal agent of anthracnose) seems to obey the gene for gene hypothesis of Flor (1947). According to this model, host/pathogen recognition involves sets of single dominant resistance genes in the host and corresponding sets of single avirulence genes in the pathogen. In bean and in other plant species, many specific resistance genes have been characterized at the genetic level; however neither the gene product nor the function of these genes is known. In order to investigate the molecular basis of the plant/pathogen recognition specificity, we intend to clone a bean resistance gene. A reverse genetics approach is under progress. It requires to locate molecular markers closely linked to the resistance gene, to establish a correlation between genetic and physical maps in the vicinity of the resistance gene, to construct a genomic library and to look for the gene of interest by chromosome walking from selected linked markers. More... »

PAGES

33-36

Book

TITLE

Mechanisms of Plant Defense Responses

ISBN

978-94-010-4761-6
978-94-011-1737-1

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-94-011-1737-1_4

DOI

http://dx.doi.org/10.1007/978-94-011-1737-1_4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1043706015


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