Multicellular Hepatocyte Spheroids for Development of Bioartificial Liver Support System and Drug Metabolism Studies View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1995

AUTHORS

A. Lazar , F. J. Wu , H. J. Mann , R. P. Remmel , F. B. Cerra , W-S. Hu

ABSTRACT

A collagen entrapment hollow fiber bioreactor employing xenogeneic hepatocytes has been developed as a short term extracorporeal bioartificial liver (BAL) support for patients in acute liver failure. Scaling up the system requires large quantities of viable and highly active cells. Hepatocytes grown as spheroids manifest higher metabolic activities for longer time periods as compared to hepatocytes cultivated as single cell cultures. A method was developed for preparation of hepatocyte spheroids on a large scale. Porcine hepatocytes when cultured under stirred conditions, form multicellular spheroids in hormonally defined culture medium within 24 hr with an efficiency of 80-90%. Morphological studies show a resemblance between cells in the spheroids and in the liver in vivo. Specific liver functions of hepatocyte spheroids such as ureagenesis, albumin production and drug metabolism were enhanced for longer culture periods as compared with single hepatocytes. Collagen entrapment of hepatocyte spheroids into a hollow fiber bioreactor is currently being evaluated as a BAL device. More... »

PAGES

1017-1023

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-94-011-0437-1_161

DOI

http://dx.doi.org/10.1007/978-94-011-0437-1_161

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1041392824


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