A New Concept of Plant In Vitro Cultivation Liquid Medium: Temporary Immersion View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1995

AUTHORS

C. Teisson , D. Alvard

ABSTRACT

The advantages of in vitro culture in a liquid medium are often counterbalanced by technical problems such as asphyxia, hyperhydricity and the need for complex apparatus. A new culture technique has been developed based on temporary immersion that reduces these disadvantages. The technique can be used with a commercial filtration unit with only minor modifications and a laboratory air pump to flush the liquid medium. The system has been successfully employed with different species (Coffea, Hevea, Musa) and different multiplication systems (microcutting, somatic embryogenesis, meristem proliferation). The quality of plant development is generally better than that obtained with traditional liquid or semi-solid medium and this can be put down to the close contact with nutrients with no deterioration in gaseous exchanges of the expiants. The temporary immersion technique can profitably replace costly biofermentors in both research and mass production. More... »

PAGES

105-110

Book

TITLE

Current Issues in Plant Molecular and Cellular Biology

ISBN

978-94-010-4135-5
978-94-011-0307-7

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-94-011-0307-7_12

DOI

http://dx.doi.org/10.1007/978-94-011-0307-7_12

DIMENSIONS

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