Ontology type: schema:Chapter
1988
AUTHORSR. Depaepe , D. Prat , X. Q. Li , P. Chetrit , E. Cousin , F. Vedel
ABSTRACTDifferent lines of Nicotiana sylvestris, a diploid autogamous species (2n =24), were used to initiate protoplast culture from leaf mesophyll cells: The original line (T line) is a pure line from the Institut des Tabacs, Bergerac, France, morphologically very homogeneous (“flat” phenotype).Diploid androgenetic lines were obtained by consecutive pollen cultures from the T line. These lines, theoretically completely homozygous (Doubled-Haploid or DH) show typical alterations of leaf morphology that we previously called “crumpling” (FR: fripé) (De Paepe et al, 1977, De Paepe et Pernès, 1978). The original line (T line) is a pure line from the Institut des Tabacs, Bergerac, France, morphologically very homogeneous (“flat” phenotype). Diploid androgenetic lines were obtained by consecutive pollen cultures from the T line. These lines, theoretically completely homozygous (Doubled-Haploid or DH) show typical alterations of leaf morphology that we previously called “crumpling” (FR: fripé) (De Paepe et al, 1977, De Paepe et Pernès, 1978). More... »
PAGES381-382
Progress in Plant Protoplast Research
ISBN
978-94-010-7754-5
978-94-009-2788-9
http://scigraph.springernature.com/pub.10.1007/978-94-009-2788-9_138
DOIhttp://dx.doi.org/10.1007/978-94-009-2788-9_138
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