Role of restriction and modification on genetic exchange in Neisseria gonorrhoeae View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1988

AUTHORS

D. C. Stein , S. T. Gregoire , A. J. Piekarowicz

ABSTRACT

Various strains of Neisseria gonorrhoeae were characterized with respect to their restriction/modification phenotype. Strain WR302 was found to lack m·NgoII but produced the following methylases activities: m·NgoI; m·NgoIII; m·NgoIV; m·NgoV and m·NgoVI. Strain Pgh3-2 contained methylase activities m·NgoI-V, but lacked m·NgoVI. It also was able to produceNgoSl (an isoschizomer ofHaeIII). Derivatives of these strains containing either plasmid pFT6 (A self transmissible R plasmid) or pFT180 were constructed. pFT180 Isolated from strain WR302 was unable to transform strain Pgh3-2 (<7.1 x 10-8), whereas pFT180 isolated from Pgh3-2 was able to transform Pgh3-2 at a frequency of 2.3 x 10-3. When WR302 grown pFT180 was methylated in vitro with m·HaeIII, this plasmid was able to transform Pgh3-2 at a frequency of 2.1 x 10-5. The restriction enzymeNgo SI was purified and found to be able to restrict strain WR302 DNA in vitro, whereas it was incapable of restricting Pgh3-2 DNA. Conjugations between WR302 (pFT6) and Pgh3-2 and Pgh3-2 (pFT6) and Pgh3-2 occurred at the same frequency (2.3 x 10-4 vs 4.0 x 10-4). The data indicate that host mediated restriction can prevent the gonococcus from acquiring DNA via transformation but that it plays no role in conjugation. More... »

PAGES

323-327

References to SciGraph publications

Book

TITLE

Gonococci and Meningococci

ISBN

978-94-010-7117-8
978-94-009-1383-7

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-94-009-1383-7_52

DOI

http://dx.doi.org/10.1007/978-94-009-1383-7_52

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1047346938


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