Ammonium Concentration Control in Fed-Batch Fermentations for the Production of Biomass and Enzymes View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1988

AUTHORS

Manoj M. Kole , Donald F. Gerson

ABSTRACT

A control system has been devised to maintain stable ammonium concentrations throughout a fed-batch fermentation. The control system is based on an ammonium gas-sensing electrode that monitors a pH adjusted effluent stream from the fermentor. The ammonium electrode (Orion 95–12) was stable throughout the fermentation period. This control system was used to study the growth of Escherichia coli and Saccharomyces cerevisiae at controlled ammonium concentrations. Apparent specific growth rates, biomass and protein production, and glucose and ammonium yield were determined.The effect of controlling ammonium concentration on growth and protease production by Bacillus subtilis in fermentors was also studied. Protease production was optimum when ammonium concentration was controlled at 5 mM. Protease production in fermentations controlled at this ammonium concentration was 1.5 times greater than in uncontrolled batch fermentations. Simultaneous control of ammonium and glucose concentrations using controllers based on an ammonium electrode and an oxygen electrode doubled protease production compared with fermentations having only ammonium control and tripled protease production compared with uncontrolled batch fermentations. The protease yield per mole of glucose and ammonium was greatest in simultaneous glucose and ammonium controlled fermentations. More... »

PAGES

94-103

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-94-009-1371-4_9

DOI

http://dx.doi.org/10.1007/978-94-009-1371-4_9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1040602155


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