Leaf disc transformation View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1989

AUTHORS

Robert B. Horsch , Joyce Fry , Nancy Hoffmann , Jeanne Neidermeyer , Stephen G. Rogers , Robert T. Fraley

ABSTRACT

Leaf disc transformation of tobacco is the paradigm for Agrobacterium-mediated transformation of plant tissues and subsequent selection and regeneration of transgenic plants. This system permits efficient gene transfer, selection and regeneration to be coupled together in a simple process. Tobacco is an excellent host for A. tumefaciens, and also responds exceedingly well in culture. While the technique is most easily practiced with tobacco, it has been applied to a number of other species (Table 1). This example will be described for tobacco, using a vector that confers kanamycin resistance, pMON200 [5]. More... »

PAGES

63-71

Book

TITLE

Plant Molecular Biology Manual

ISBN

978-94-010-6918-2
978-94-009-0951-9

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-94-009-0951-9_5

DOI

http://dx.doi.org/10.1007/978-94-009-0951-9_5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1006939580


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