Transcription regulation of a Kunitz chymotrypsin inhibitor gene which is expressed abundantly in seeds and tuberous roots of winged bean View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1997

AUTHORS

Yoichi Sakata , Sanae Tamura , Yasuyo Yamazaki , Yoichi Ohdaira , Tomomasa Kuwano , Noriko Takashima , Hiroshi Takenaga , Yoshiki Habu , Satoshi Naito

ABSTRACT

A winged bean Kunitz-type chymotrypsin inhibitor (WCI) accumulates abundantly in developing embryos and tuberous roots, and also in a small amount in stems. Promoter activity of the 1.0 kbp upstream region of the WCI-3b gene, which encodes a major WCI protein in seeds, was analyzed in transgenic tobacco plants. The 1.0 kbp upstream region directed strong expression of a reporter gene (ß-glucuronidase; GUS) in developing seeds of the transgenic tobacco. The GUS activity was also detected in stems, although no significant GUS activity was observed in leaves. Histochemical analysis revealed that the GUS gene was mainly expressed in embryos and in phloem of stems. These results suggested that the transcription regulation plays an important role in the expression of the WCI-3b gene in embryos and in phloem of stems. The cis-elements promoting transcription of the WCI-3b gene in these cells are included within the 1.0 kbp upstream region. More... »

PAGES

219-220

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-94-009-0047-9_57

DOI

http://dx.doi.org/10.1007/978-94-009-0047-9_57

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1049735423


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