Engineering Principles and Cell Culture Performance of Orbitally Shaken Cylindrical Bioreactors View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2011-07-25

AUTHORS

S. Tissot , X. Zhang , M. Stettler , D. De Sanctis , M. Perone , C. Bürki , M. De Jesus , M. Farhat , M. Discacciati , O.W. Reif , M. Kühner , Lucia Baldi , David L. Hacker , A. Quarteroni , Florian M. Wurm

ABSTRACT

We are investigating the use of orbitally shaken cylindrical containers for the cultivation of suspension-adapted mammalian cells at working volumes from a few mLs to 1000 L. Here we present data on mixing, oxygen transfer, and cell culture performance in these bioreactors. kLa values for volumes up to 1000 L and mixing times for volumes up to 400 L were measured at shaking speeds suitable for mammalian cell culture. Without pH or oxygen control, cell densities equivalent to fully controlled bioreactors were obtained at scales up to 100 L in a 200-L bioreactor. A 500-L culture run in a 2000-L orbitally shaken bioreactor (non-optimized prototype) reached 85% of the maximal cell density and 65% of the recombinant protein concentration compared to 10-mL cultures in 50-mL Tubespins. Only air was used as the oxygen source at all the scales, demonstrating that oxygen transfer through the free surface in orbitally shaken bioreactor is sufficient for mammalian cell cultures. Overall, our data indicate that orbitally shaken bioreactors are an interesting alternative to conventional stirred-tank bioreactors. More... »

PAGES

407-412

References to SciGraph publications

Book

TITLE

Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009

ISBN

978-94-007-0883-9
978-94-007-0884-6

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-94-007-0884-6_67

DOI

http://dx.doi.org/10.1007/978-94-007-0884-6_67

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1052308425


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