An Improved Clone Selection Method View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2012

AUTHORS

Uros Jamnikar , Marjanca Blas , Dominik Gaser , Jerica Rozman-Pungercar , Andrej Francky

ABSTRACT

For commercial production of recombinant proteins and antibodies it is very important to obtain high producing cell clones. Due to this demand it is inevitable to introduce cell line development methods that enable screening of more clones and increase chance to isolate clones with best characteristics. It is hard and time consuming to find high producing clones using standard cloning methods as limited dilution. In most cases there are no resources to isolate and analyze more than few 100 clones. ClonePix FL (CP-FL, Genetix) technology substantially increases the number of screened clones in the selected pool before expansion in microtiter plates. In this study we wanted to compare productivity of clones obtained with limited dilution method and those obtained with ClonePix FL technology. More... »

PAGES

57-60

Book

TITLE

Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009

ISBN

978-94-007-0883-9
978-94-007-0884-6

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-94-007-0884-6_10

DOI

http://dx.doi.org/10.1007/978-94-007-0884-6_10

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1024946312


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