Screening of Natural Compounds Affecting Type I Interferon Signalling View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2010-06-02

AUTHORS

M. Bollati-Fogolín , M. Oggero , S. Mirazo , R. Frank , R. Kratje , W. Müller

ABSTRACT

Interferons are a family of cytokine mediators involved in the cellular immune response, i.e. against viral infections or amplifying antigen presentation to specific T cells. Recombinant human IFN-alpha±2a and IFN-alpha±2b are used for the treatment of several diseases, while recombinant forms of IFN-beta1a and IFN-beta1b are only certified for the treatment of multiple sclerosis. Recently, we have reported the generation of a novel cell-based assay to quantify murine IFNs. The assay consists of a murine type I IFN induction phase, which drives the expression of Cre recombinase from the Mx/RAGE cell line. Cre-recombinase mediated deletion of loxP flanked resistance cassette leads to EGFP expression. The read-out of the system consists of the detection of EGFP positive cells by fluorescence activated cell sorting. One of the advantages of this method is that allows the design of robust high through put screening assays. Taking these together, in the present work, we aim to select natural compounds able to induce or interfere with murine type I IFN signaling. Screening of a library composed of 115 natural compounds from the myxococcal metabolite collection of the HZI yielded twelve compounds showing a synergistic effect with more than three-fold increase on the mIFN activity. One of these hits, Compound A was further characterized. More... »

PAGES

485-487

Book

TITLE

Cells and Culture

ISBN

978-90-481-3418-2
978-90-481-3419-9

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-90-481-3419-9_86

DOI

http://dx.doi.org/10.1007/978-90-481-3419-9_86

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1053275962


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Immunology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "National University of the Littoral", 
          "id": "https://www.grid.ac/institutes/grid.10798.37", 
          "name": [
            "Cell Biology Unit, Institut Pasteur de Montevideo, Montevideo, Uruguay", 
            "Laboratorio de Cultivos Celulares. Facultad de Bioqu\u00edmica y Ciencias, Biol\u00f3gicas. UNL. C.C. 242, Ciudad Universitaria (S3000ZAA) Santa Fe, Pcia, Santa Fe, Argentina"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bollati-Fogol\u00edn", 
        "givenName": "M.", 
        "id": "sg:person.01034422411.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034422411.10"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National University of the Littoral", 
          "id": "https://www.grid.ac/institutes/grid.10798.37", 
          "name": [
            "Laboratorios de Cultivos Celulares, Universidad Nacional del Litoral, Santa Fe, Argentina"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Oggero", 
        "givenName": "M.", 
        "id": "sg:person.01156276723.63", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01156276723.63"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut Pasteur de Montevideo", 
          "id": "https://www.grid.ac/institutes/grid.418532.9", 
          "name": [
            "Cell Biology Unit, Institut Pasteur de Montevideo, Montevideo, Uruguay"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mirazo", 
        "givenName": "S.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Department of Chemical Biology, Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Frank", 
        "givenName": "R.", 
        "id": "sg:person.01220637525.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01220637525.11"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National University of the Littoral", 
          "id": "https://www.grid.ac/institutes/grid.10798.37", 
          "name": [
            "Laboratorio de Cultivos Celulares. Facultad de Bioqu\u00edmica y Ciencias, Biol\u00f3gicas. UNL. C.C. 242, Ciudad Universitaria (S3000ZAA) Santa Fe, Pcia, Santa Fe, Argentina"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kratje", 
        "givenName": "R.", 
        "id": "sg:person.01064706510.72", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01064706510.72"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Department of Experimental Immunology Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "M\u00fcller", 
        "givenName": "W.", 
        "id": "sg:person.01261504775.94", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261504775.94"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2010-06-02", 
    "datePublishedReg": "2010-06-02", 
    "description": "Interferons are a family of cytokine mediators involved in the cellular immune response, i.e. against viral infections or amplifying antigen presentation to specific T cells. Recombinant human IFN-alpha\u00b12a and IFN-alpha\u00b12b are used for the treatment of several diseases, while recombinant forms of IFN-beta1a and IFN-beta1b are only certified for the treatment of multiple sclerosis. Recently, we have reported the generation of a novel cell-based assay to quantify murine IFNs. The assay consists of a murine type I IFN induction phase, which drives the expression of Cre recombinase from the Mx/RAGE cell line. Cre-recombinase mediated deletion of loxP flanked resistance cassette leads to EGFP expression. The read-out of the system consists of the detection of EGFP positive cells by fluorescence activated cell sorting. One of the advantages of this method is that allows the design of robust high through put screening assays. Taking these together, in the present work, we aim to select natural compounds able to induce or interfere with murine type I IFN signaling. Screening of a library composed of 115 natural compounds from the myxococcal metabolite collection of the HZI yielded twelve compounds showing a synergistic effect with more than three-fold increase on the mIFN activity. One of these hits, Compound A was further characterized.", 
    "editor": [
      {
        "familyName": "Noll", 
        "givenName": "Thomas", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-90-481-3419-9_86", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-90-481-3418-2", 
        "978-90-481-3419-9"
      ], 
      "name": "Cells and Culture", 
      "type": "Book"
    }, 
    "name": "Screening of Natural Compounds Affecting Type I Interferon Signalling", 
    "pagination": "485-487", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1053275962"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-90-481-3419-9_86"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "fdc3e894cc589efe3af3561e71c50e8cba2257640eeb1791b5e63627191078ef"
        ]
      }
    ], 
    "publisher": {
      "location": "Dordrecht", 
      "name": "Springer Netherlands", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-90-481-3419-9_86", 
      "https://app.dimensions.ai/details/publication/pub.1053275962"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2019-04-16T08:00", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000359_0000000359/records_29191_00000002.jsonl", 
    "type": "Chapter", 
    "url": "https://link.springer.com/10.1007%2F978-90-481-3419-9_86"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-90-481-3419-9_86'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-90-481-3419-9_86'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-90-481-3419-9_86'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-90-481-3419-9_86'


 

This table displays all metadata directly associated to this object as RDF triples.

108 TRIPLES      22 PREDICATES      26 URIs      19 LITERALS      8 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-90-481-3419-9_86 schema:about anzsrc-for:11
2 anzsrc-for:1107
3 schema:author Nc6eb5b0612394afa9c515686ca93555b
4 schema:datePublished 2010-06-02
5 schema:datePublishedReg 2010-06-02
6 schema:description Interferons are a family of cytokine mediators involved in the cellular immune response, i.e. against viral infections or amplifying antigen presentation to specific T cells. Recombinant human IFN-alpha±2a and IFN-alpha±2b are used for the treatment of several diseases, while recombinant forms of IFN-beta1a and IFN-beta1b are only certified for the treatment of multiple sclerosis. Recently, we have reported the generation of a novel cell-based assay to quantify murine IFNs. The assay consists of a murine type I IFN induction phase, which drives the expression of Cre recombinase from the Mx/RAGE cell line. Cre-recombinase mediated deletion of loxP flanked resistance cassette leads to EGFP expression. The read-out of the system consists of the detection of EGFP positive cells by fluorescence activated cell sorting. One of the advantages of this method is that allows the design of robust high through put screening assays. Taking these together, in the present work, we aim to select natural compounds able to induce or interfere with murine type I IFN signaling. Screening of a library composed of 115 natural compounds from the myxococcal metabolite collection of the HZI yielded twelve compounds showing a synergistic effect with more than three-fold increase on the mIFN activity. One of these hits, Compound A was further characterized.
7 schema:editor N49e4405c6b4344d8be7b1c054c8cb40a
8 schema:genre chapter
9 schema:inLanguage en
10 schema:isAccessibleForFree false
11 schema:isPartOf N1483d15791b7476db592ea1b2eca5c84
12 schema:name Screening of Natural Compounds Affecting Type I Interferon Signalling
13 schema:pagination 485-487
14 schema:productId N1d0546204a7c46f4883ab3201bd62acf
15 Nb882e2f4001348e3bf74e94ea57c7a2c
16 Nf4578271e0ca434483629fd4a5607931
17 schema:publisher N3da9957671f445308cf216d28c03a3ae
18 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053275962
19 https://doi.org/10.1007/978-90-481-3419-9_86
20 schema:sdDatePublished 2019-04-16T08:00
21 schema:sdLicense https://scigraph.springernature.com/explorer/license/
22 schema:sdPublisher N8ee0cf597c574394bf71023e554c4b92
23 schema:url https://link.springer.com/10.1007%2F978-90-481-3419-9_86
24 sgo:license sg:explorer/license/
25 sgo:sdDataset chapters
26 rdf:type schema:Chapter
27 N1483d15791b7476db592ea1b2eca5c84 schema:isbn 978-90-481-3418-2
28 978-90-481-3419-9
29 schema:name Cells and Culture
30 rdf:type schema:Book
31 N1b01ea67439146949e1be2fbed9460e1 schema:name Department of Chemical Biology, Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany
32 rdf:type schema:Organization
33 N1ce241e80c084e5fadaa9fd27e9dfe18 rdf:first sg:person.01156276723.63
34 rdf:rest N98cc3e2dfc3d4f63931f242b04687f0b
35 N1d0546204a7c46f4883ab3201bd62acf schema:name readcube_id
36 schema:value fdc3e894cc589efe3af3561e71c50e8cba2257640eeb1791b5e63627191078ef
37 rdf:type schema:PropertyValue
38 N3930ad9a4f214050a7fc66f3be259a61 schema:familyName Noll
39 schema:givenName Thomas
40 rdf:type schema:Person
41 N3da9957671f445308cf216d28c03a3ae schema:location Dordrecht
42 schema:name Springer Netherlands
43 rdf:type schema:Organisation
44 N49e4405c6b4344d8be7b1c054c8cb40a rdf:first N3930ad9a4f214050a7fc66f3be259a61
45 rdf:rest rdf:nil
46 N8ee0cf597c574394bf71023e554c4b92 schema:name Springer Nature - SN SciGraph project
47 rdf:type schema:Organization
48 N9045ff79284e4753b51a2f2d0f6f3d92 rdf:first sg:person.01220637525.11
49 rdf:rest Nafb0926e95974181936f83cf0651f00e
50 N98cc3e2dfc3d4f63931f242b04687f0b rdf:first N9fe5c05484bb4e61b738df932eb2e2b1
51 rdf:rest N9045ff79284e4753b51a2f2d0f6f3d92
52 N9fe5c05484bb4e61b738df932eb2e2b1 schema:affiliation https://www.grid.ac/institutes/grid.418532.9
53 schema:familyName Mirazo
54 schema:givenName S.
55 rdf:type schema:Person
56 Nafb0926e95974181936f83cf0651f00e rdf:first sg:person.01064706510.72
57 rdf:rest Nb4a05acb8cf14c51b651eb9e813ebc6a
58 Nb4a05acb8cf14c51b651eb9e813ebc6a rdf:first sg:person.01261504775.94
59 rdf:rest rdf:nil
60 Nb882e2f4001348e3bf74e94ea57c7a2c schema:name dimensions_id
61 schema:value pub.1053275962
62 rdf:type schema:PropertyValue
63 Nc6eb5b0612394afa9c515686ca93555b rdf:first sg:person.01034422411.10
64 rdf:rest N1ce241e80c084e5fadaa9fd27e9dfe18
65 Nd35ec834a2c74a8abcc59d65bbd0efcc schema:name Department of Experimental Immunology Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany
66 rdf:type schema:Organization
67 Nf4578271e0ca434483629fd4a5607931 schema:name doi
68 schema:value 10.1007/978-90-481-3419-9_86
69 rdf:type schema:PropertyValue
70 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
71 schema:name Medical and Health Sciences
72 rdf:type schema:DefinedTerm
73 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
74 schema:name Immunology
75 rdf:type schema:DefinedTerm
76 sg:person.01034422411.10 schema:affiliation https://www.grid.ac/institutes/grid.10798.37
77 schema:familyName Bollati-Fogolín
78 schema:givenName M.
79 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034422411.10
80 rdf:type schema:Person
81 sg:person.01064706510.72 schema:affiliation https://www.grid.ac/institutes/grid.10798.37
82 schema:familyName Kratje
83 schema:givenName R.
84 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01064706510.72
85 rdf:type schema:Person
86 sg:person.01156276723.63 schema:affiliation https://www.grid.ac/institutes/grid.10798.37
87 schema:familyName Oggero
88 schema:givenName M.
89 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01156276723.63
90 rdf:type schema:Person
91 sg:person.01220637525.11 schema:affiliation N1b01ea67439146949e1be2fbed9460e1
92 schema:familyName Frank
93 schema:givenName R.
94 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01220637525.11
95 rdf:type schema:Person
96 sg:person.01261504775.94 schema:affiliation Nd35ec834a2c74a8abcc59d65bbd0efcc
97 schema:familyName Müller
98 schema:givenName W.
99 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261504775.94
100 rdf:type schema:Person
101 https://www.grid.ac/institutes/grid.10798.37 schema:alternateName National University of the Littoral
102 schema:name Cell Biology Unit, Institut Pasteur de Montevideo, Montevideo, Uruguay
103 Laboratorio de Cultivos Celulares. Facultad de Bioquímica y Ciencias, Biológicas. UNL. C.C. 242, Ciudad Universitaria (S3000ZAA) Santa Fe, Pcia, Santa Fe, Argentina
104 Laboratorios de Cultivos Celulares, Universidad Nacional del Litoral, Santa Fe, Argentina
105 rdf:type schema:Organization
106 https://www.grid.ac/institutes/grid.418532.9 schema:alternateName Institut Pasteur de Montevideo
107 schema:name Cell Biology Unit, Institut Pasteur de Montevideo, Montevideo, Uruguay
108 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...