Screening of Natural Compounds Affecting Type I Interferon Signalling View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2010-06-02

AUTHORS

M. Bollati-Fogolín , M. Oggero , S. Mirazo , R. Frank , R. Kratje , W. Müller

ABSTRACT

Interferons are a family of cytokine mediators involved in the cellular immune response, i.e. against viral infections or amplifying antigen presentation to specific T cells. Recombinant human IFN-alpha±2a and IFN-alpha±2b are used for the treatment of several diseases, while recombinant forms of IFN-beta1a and IFN-beta1b are only certified for the treatment of multiple sclerosis. Recently, we have reported the generation of a novel cell-based assay to quantify murine IFNs. The assay consists of a murine type I IFN induction phase, which drives the expression of Cre recombinase from the Mx/RAGE cell line. Cre-recombinase mediated deletion of loxP flanked resistance cassette leads to EGFP expression. The read-out of the system consists of the detection of EGFP positive cells by fluorescence activated cell sorting. One of the advantages of this method is that allows the design of robust high through put screening assays. Taking these together, in the present work, we aim to select natural compounds able to induce or interfere with murine type I IFN signaling. Screening of a library composed of 115 natural compounds from the myxococcal metabolite collection of the HZI yielded twelve compounds showing a synergistic effect with more than three-fold increase on the mIFN activity. One of these hits, Compound A was further characterized. More... »

PAGES

485-487

Book

TITLE

Cells and Culture

ISBN

978-90-481-3418-2
978-90-481-3419-9

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-90-481-3419-9_86

DOI

http://dx.doi.org/10.1007/978-90-481-3419-9_86

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1053275962


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